欧李不同级次根的解剖结构及生理特性研究

发布时间：2018-11-12 07:44

【摘要】：为探讨欧李不同级次根的解剖结构和生理特性,本试验以5年生实生欧李为试材,采用根序分级法对欧李根系进行分级,用徒手切片法观测其横切面结构,用铬酸-硝酸离析法对其进行离析并观测其导管分子结构,用树脂复型法制作导管模型并用扫描电镜拍照;用TTC法、Lorry法、茚三酮比色等方法测定不同级次根的根系活力、蛋白质含量、游离氨基酸含量、SOD活性、NRA活性等。试验结果表明:1.欧李的1-2级根是初生根,具有薄壁组织,薄壁组织率高达88.44%和80.61%;3-6级根为次生根,有周皮,周皮厚度分别为48.988μm、94.073μm、143.057μm、186.833μm,对外界胁迫的抵抗能力随级次的升高而升高。2.欧李的1-2级根的导管直径分别为17.815μm和15.260μm,远小于3-6级根。其导管密度分别为1172.330个·mm-2和836.000个·mm-2,无尾导管的比率为55.25%和46.47%、孔纹导管的比率最高分别为88.46%和90.38%,均高于3-6级根。3.欧李1-2级根的质量仅占根系的4.18%。但1-2级根代谢活跃,其根系活力高达186.252μg·g~(-1)·h~(-1)、活跃吸收面积/总吸收面积的值高达83.62%,均显著高于3-6级根。4.欧李3级根的蛋白质含量最高,为18.33 mg·g~(-1)。3-4级根中N代谢比较活跃。根系的蛋白质含量与谷氨酰胺合成酶、谷氨酸脱氢酶活性呈显著正相关。5.欧李1-2级根的SOD活性高达222.220 U·g~(-1)·min~(-1)、POD活性为5.791U·g~(-1)·min~(-1)、CAT活性为13.334 U·g~(-1)·min~(-1),均高于3-6级根。但3-6级根的可溶性糖、淀粉、纤维素、游离氨基酸和脯氨酸等营养物质的含量高于1-2级根,且两两之间呈显著或极显著正相关。
[Abstract]:In order to study the anatomical structure and physiological characteristics of different order roots of Euchamnus oleifera, the root system was classified by the method of root sequence classification, and the cross section structure was observed by the method of bare hand slicing. Chromic acid-nitric acid segregation method was used to isolate and observe the molecular structure of the conduit. The model of the catheter was made by resin replica method and the photograph was taken by scanning electron microscope (SEM). The root activity, protein content, free amino acid content, SOD activity and NRA activity were determined by TTC, Lorry and ninhydrin colorimetry. The results show that: 1. The 1-2 grade roots were primary rooting and had parenchyma tissue. The percentage of parenchyma tissue was 88.44% and 80.61% respectively. The 3-6 grade roots were secondary roots with pericarp. The thickness of pericarp was 48.988 渭 m ~ 94.073 渭 m ~ (143.057) 渭 m ~ (-1) ~ 186.833 渭 m, respectively. The catheter diameters of the 1-2 order roots were 17.815 渭 m and 15.260 渭 m, respectively, which were much smaller than the 3-6 grade roots. The ducts density of 1172.330 mm-2 and 836.000 mm-2, caudal catheters were 55.25% and 46.47 respectively. The highest rates of perforated ducts were 88.46% and 90.38% respectively, which were higher than those of 3-6 caudal catheters. The root quality of grade 1-2 was only 4.18% of the root system. However, the root metabolism was active in grade 1-2, the root activity was up to 186.252 渭 g g ~ (-1) h ~ (-1), and the ratio of active absorption area to total absorption area was 83.62, which was significantly higher than that of 3-6 grade roots. The highest protein content was 18.33 mg g ~ (-1) in the roots of grade 3, and the metabolism of N was more active in the roots of grade 3-4. The protein content in roots was positively correlated with glutamine synthetase and glutamate dehydrogenase activity. The SOD activity of the roots of grade 1-2 was up to 222.220 U g ~ (-1) min~ (-1), POD) and the activity of min~ (-1), CAT) was 13.334 U g ~ (-1) min~ (-1). All of them were higher than 3-6 grade roots. However, the contents of soluble sugar, starch, cellulose, free amino acid and proline in 3-6 grade roots were higher than those in 1-2 grade roots, and there was a significant or extremely significant positive correlation between the content of soluble sugar, starch, cellulose, free amino acids and proline.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S662.5

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