电场处理糜子种子对其萌发期和苗期抗旱性的影响及时效性

发布时间：2018-12-15 05:52

【摘要】：用不同电场条件(电场频率为4～6kHz,电场强度为2～5kV/cm,处理时间为5min和l0min)对糜子种子进行处理后,采用浓度为16%的聚乙二醇6000溶液模拟干旱胁迫,实验测定糜子萌发期发芽率、胚根长、胚芽长和根干重等,筛选显著提高糜子种子抗旱性的电场处理条件;利用筛选所得电场条件处理糜子种子,测定干旱胁迫下幼苗的株高、根长、叶绿素含量和超氧化物歧化酶(SOD)活力等指标,分析电场处理条件对幼苗抗旱性的影响;用筛选出的电场条件处理糜子种子后,分别在当天,贮存7天,14天和21天进行萌发实验,测定干旱胁迫下糜子种子的发芽势和发芽率,探究电场对糜子影响的时效性。本实验测定不同电场频率,不同电场强度及不同处理时间对植物生长影响的规律,旨在找出显著提高植物抗旱性的电场条件,为电生物技术在农业生产中的开发利用提供理论和实验依据。实验结果表明:与自然对照组比较,干旱胁迫使糜子种子发芽率、胚根长及胚芽长均降低,下降幅度分别为17.84%、65.58%及67.41%,胚根干重与胚芽干重则显著增加,增长幅度分别为34.63%与23.25%;电场处理糜子种子,可降低干旱胁迫对发芽率、胚根长及胚芽长的不利影响,使得胚根干重和胚芽干重整体保持上升趋势,胚根干重和胚芽干重增长幅度最高可达58.45%和44.02%。电场处理对干旱胁迫下糜子幼苗的株高、根长、根鲜重与根干重有不同程度的促进作用,它们相对干旱胁迫对照组的增长率分别为25.33%、37.73%、217.19%和158.54%。实验还发现,电场处理糜子种子的生物效应存在时效性,糜子种子经电场处理后,贮存不同时间,糜子种子的发芽势和发芽率产生不同变化,随着贮存时间的推移,电场处理糜子种子产生的影响逐渐消失。
[Abstract]:The seeds were treated with different electric field conditions (electric field frequency of 4 ~ 6 kHz, electric field intensity of 2 ~ 5 kV / cm, treatment time of 5min and l0min). After treatment, 16% polyethylene glycol 6000 solution was used to simulate drought stress. The germination rate, radicle length, germ length and root dry weight were determined in order to select the electric field treatment conditions to improve the drought resistance of millet seeds. The plant height, root length, chlorophyll content and (SOD) activity of superoxide dismutase (SOD) were measured under drought stress, and the effects of electric field treatment on drought resistance were analyzed. The germinating experiments were carried out on the same day, 7 days, 14 days and 21 days after the treatment with the selected electric field conditions. The germinating potential and germination rate of the millet seeds under drought stress were measured, and the time effect of electric field on the mildew was investigated. The effects of different electric field frequencies, different electric field intensity and different treatment time on plant growth were determined in order to find out the electric field conditions that could significantly improve the drought resistance of plants. It provides theoretical and experimental basis for the development and utilization of electric biotechnology in agricultural production. The results showed that: compared with the natural control group, drought stress decreased the germination rate, radicle length and germ length of mycorrhiza hygrophylla, which decreased by 17.84% and 67.41%, respectively. The dry weight of radicle and dry weight of germ increased significantly. The increase was 34.63% and 23.25% respectively. When treated with electric field, the adverse effects of drought stress on germination rate, radicle length and germ length could be reduced, and the dry weight of radicle and dry weight of embryo kept a rising trend as a whole. The maximum increase range of dry weight of radicle and embryo was 58.45% and 44.02% respectively. The plant height, root length, root fresh weight and root dry weight were promoted by electric field treatment, and the growth rates of them were 25.33 ~ 37.73% and 158.54%, respectively. It was also found that the biological effects of electric field treatment were time-dependent. After stored in different time, the germination potential and germination rate of mycorrhiza mycorrhizae seeds varied with the storage time. The effect of electric field treatment on mildew seeds gradually disappeared.
【学位授予单位】：内蒙古大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S516

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