雪柑根系缺镁响应microRNA分离和鉴定

发布时间：2019-03-31 13:20

【摘要】：缺镁(Mg)多发生在强酸性的沙质土壤中,而柑橘主要生长在酸性或强酸性的土壤当中。缺镁会降低一些柑橘园的果实品质和产量。基于此,我们率先采用Illumina sequencing对缺Mg和供Mg充足的雪柑根系构成的2个RNA文库进行了高能量测序,旨在获得一些可能与雪柑根系耐缺Mg相关的miRNA。通过高能量测序,本研究从缺Mg雪柑根系中分离得到了 101个上调和69个下调miRNA。分析结果表明,雪柑根系对缺Mg的适应性响应可能有以下几个方面:(a)抑制根系呼吸,以及通过诱导miR158和miR2919的表达抑制呼吸相关基因的表达;(b)通过下调相关miRNA的表达来提高其抗氧化系统(miR780、miR6190、miR1044、miR5261和miR1151)和对低磷的适应性(miR6190);(c)通过改变miR6190、miR6485、miR1044、miR5029和miR3437来激活与运输相关基因的表达;(d)降低miR394、miR1044、miR5261、iR1151和miR5029的表达,从而提高蛋白泛素化;(e)通过下调miR5261和miR6485和上调miR158的表达来维持根部的生长;(f)通过调控miR5176和miR6485(miR6028、miR190、miR6485、miR5621、miR160和miR7708)启动DNA修复(转录调节)。此外,与根信号传导相关的一些缺Mg响应miRNA也可能在雪柑对缺Mg的适应性响应中起作用。缺Mg响应miRNA可能在根信号传导中起作用,从而提高雪柑对缺Mg适应性。总之,本研究分离鉴定了一些与缺Mg耐性相关的新的缺Mg响应 miRNA(miR5261、miR158、miR6190、miR6485、miR1151 和 miR1044)。这些结果揭示了在植物营养缺乏的适应性方面miRNAs发挥作用的一些新的线索。
[Abstract]:Magnesium deficiency (Mg) mostly occurs in sandy soil with strong acidity, while citrus mainly grows in acid or strong acid soil. Magnesium deficiency reduces fruit quality and yield in some citrus orchards. Based on this, we first used Illumina sequencing to sequence two RNA libraries composed of Mg-deficient and Mg-rich roots of Xuegan, in order to obtain some miRNA. which may be related to Mg tolerance of Xuegan root. The purpose of this study is to obtain some miRNA. libraries which may be related to the Mg tolerance of Xuegan root. By high energy sequencing, 101 up-regulated and 69 down-regulated miRNA. were isolated from the roots of Mg-deficient snow orange. The results showed that the adaptive response of Xuegan root to Mg deficiency might have the following aspects: (a) inhibited root respiration and inhibited the expression of respiratory-related genes by inducing the expression of miR158 and miR2919. (b) enhanced its antioxidant system (miR780,miR6190,miR1044,miR5261 and miR1151) and its adaptation to low phosphorus by down-regulating the expression of associated miRNA (miR6190); (c) by changing miR6190,miR6485,miR1044,miR5029 and miR3437 to activate the expression of transport-related genes. (d) decreased the expression of miR394,miR1044,miR5261,iR1151 and miR5029, thus increased the expression of ubiquitin; (e) to maintain root growth by down-regulating the expression of miR5261 and miR6485 and up-regulating the expression of miR158. (f) initiates DNA repair (transcriptional regulation) by regulating miR5176 and miR6485 (miR6028,miR190,miR6485,miR5621,miR160 and miR7708). In addition, some lack of Mg response miRNA associated with root signal transduction may also play a role in the adaptive response of Xuegan to Mg deficiency. The lack of Mg response miRNA may play a role in root signal transduction, thus improving the adaptability of Xuequan to Mg deficiency. In conclusion, we isolated and identified some new Mg-deficient response miRNA (miR5261,miR158,miR6190,miR6485,miR1151 and miR1044) related to lack of Mg tolerance. These results reveal some new clues to the role of miRNAs in the adaptation of plant nutrition deficiency.
【学位授予单位】：福建农林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S666.1

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