氟对成纤维细胞c-fos、c-jun表达的影响及意义

发布时间：2018-02-26 03:08

本文关键词： c-fos c-jun 成纤维细胞株(L929) 成骨细胞　出处：《吉林大学》2007年硕士论文　论文类型：学位论文

【摘要】： 体内、外实验及转基因动物研究发现,严格调控c-fos的表达对正常骨代谢很重要。我们曾发现氟中毒大鼠脊椎骨的c-fos、c-jun从mRNA到蛋白表达均有增加,体外培养成骨样细胞染氟后也得出类似结果。提示c-fos、c-jun基因表达在氟骨症发生方面可能具有重要作用。本研究观察氟对骨周软组织中重要细胞成分—成纤维细胞(fibroblast,FB)中c-fos、c-jun mRNA和蛋白表达的影响,并探讨c-fos、c-jun基因表达改变在FB成骨表型改变方面的作用。实验将FB分为对照组和6个加氟组(0.0001、0.001、0.1、1、10和20 mg/L),在5个加氟时间段(2、4、24、48和72 h)应用RT-PCR方法、酶联免疫吸附(ELISA)法和免疫组化方法(IHC)检测c-fos、c-jun mRNA表达和蛋白含量的变化。结果显示,各加氟组在各时间段均可明显刺激FB c-Fos蛋白的表达,染氟48 h FB c-fos mRNA表达呈上升趋势;c-jun mRNA和蛋白的变化规律性不明显。同时观察相同实验条件下染氟成骨细胞(osteoblast,OB)中c-fos和c-jun的表达,发现在c-fos表达增强方面FB和OB对氟的刺激具有相似的反应。上述结果提示1.染氟FB和OB中c-jun变化规律不明显可能和实验条件有关;2.氟刺激FB中c-fos表达增强,可能在促进FB成骨功能异常发挥,进而导致氟骨症骨周化骨的发生中有重要作用;3.从一个侧面证明在过量氟的刺激下,FB已具有与OB相似的细胞表型。
[Abstract]:In vivo, in vitro experiments and in transgenic animals, we found that strict regulation of c-fos expression is very important for normal bone metabolism. We have found that c-fos-c-jun expression in vertebrae of fluorosis rats has increased from mRNA to protein. The results showed that c-fossil-c-jun gene expression may play an important role in the occurrence of osteofluorosis. In this study, we observed the effect of fluoride on the expression of c-fos-c-jun mRNA and protein in fibroblast fibroblast FB, an important cell component in periosseous soft tissue. To investigate the role of c-fos-c-jun gene expression in osteoblastic phenotype change of FB, FB was divided into two groups: control group and six fluorine-added groups. The RT-PCR method was used in 10 and 20 mg / L of FB. Enzyme linked immunosorbent assay (Elisa) and immunohistochemical method (IHC) were used to detect the expression of c-jun mRNA and protein in c-fossi. The expression of c-fos mRNA increased at 48 h after fluoride exposure. The changes of c-jun mRNA and protein were not obvious. The expression of c-fos and c-jun in osteoblastus OBof osteoblasts exposed to fluoride under the same experimental conditions was observed at the same time. It was found that FB and OB had similar responses to the stimulation of fluorine on the expression of c-fos. The above results suggested that 1. The changes of c-jun in FB and OB may not be related to the experimental conditions. It may play an important role in promoting the abnormal function of FB osteogenesis and then leading to the occurrence of periosseous bone in osteofluorosis. It is proved from one side that FB has a cell phenotype similar to that of OB under the stimulation of excessive fluoride.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

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