复发性口腔溃疡家兔模型补充超氧化物岐化酶的作用

发布时间：2018-03-03 07:49

  本文选题：口腔溃疡　切入点：超氧化物岐化酶　出处：《中国组织工程研究》2014年49期 　论文类型：期刊论文

【摘要】：背景:复发性阿弗他溃疡的具体发病机制目前医学界尚无定论,其中体内氧自由基的增多是引起复发性口腔溃疡反复发作的原因之一。目的:观察超氧化物歧化酶对复发性阿弗他溃疡治疗效果的影响。方法:在24只复发性阿弗他溃疡家兔模型建立后,按随机数字表法分为3组。模型对照组作为对比不做处理;阴性对照组家兔每天局部碘甘油擦拭;超氧化物歧化酶组家兔每天进行超氧化物歧化酶生物胶囊溶液灌胃,溃疡局部用碘甘油进行擦拭。1周后切取口腔溃疡病变组织进行对比研究。各组家兔造模后,每隔3 d上午同一时间兔耳静脉采血,流式细胞仪分析T细胞亚群CD3+、CD4+、CD8+以及CD3+/CD8+变化。结果与结论:当溃疡病变形成后,模型对照组和阴性对照组家兔外周血中细胞CD3+、CD4+、CD4+/CD8+的比值经过统计均明显低于正常,而CD8+细胞数远远大于正常数值。超氧化物歧化酶组灌服超氧化物歧化酶生物胶囊后,CD3+、CD4+细胞数开始逐渐升高,而CD8+细胞数则相反呈下降趋势。提示应用氧自由基的天然清除剂超氧化物歧化酶就能有效清除溃疡面中过量的氧自由基,从而达到临床上治疗复发性阿弗他溃疡的目的。
[Abstract]:Background: the specific pathogenesis of recurrent aphthous ulcer is still unknown in the medical field. The increase of oxygen free radicals in the body is one of the causes of recurrent recurrent oral ulcer. Objective: to observe the effect of superoxide dismutase on the treatment of recurrent aphthous ulcer. After the establishment of the rabbit model of fatta ulcer, The model control group was not treated as a control group; the negative control group was given daily iodine glycerol swab; and the superoxide dismutase group was given intragastric administration of superoxide dismutase biological capsule solution every day. The lesion tissues of oral ulcer were removed with iodine glycerine for 1 week and the blood was collected from the ear vein at the same time every 3 days in the morning after each rabbit model was made. Flow cytometry was used to analyze the changes of CD4 / CD8 and CD3 / CD8 in T cell subsets. Results and conclusion: the ratio of CD4 / CD8 in peripheral blood of model control group and negative control group was significantly lower than that of normal control group. However, the number of CD8 cells was much larger than the normal value, and the number of CD3 + CD4 cells in the superoxide dismutase group began to increase gradually after the superoxide dismutase group was administered with biological capsules of superoxide dismutase. The number of CD8 cells decreased on the contrary, suggesting that superoxide dismutase (SOD), a natural scavenger of oxygen free radicals, could effectively scavenge excess oxygen free radicals in the ulcer surface, thus achieving the purpose of clinical treatment of recurrent aphthous ulcer.
【作者单位】： 佳木斯大学附属口腔医院;佳木斯大学附属第一医院;
【基金】：黑龙江省卫生厅项目(2013215) 2014年度黑龙江省教育厅科学技术研究项目计划(12541784)~~
【分类号】：R-332;R781.5
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本文编号：1560158