骨形态发生蛋白-2诱导骨髓间质干细胞成软骨分化的实验研究

发布时间：2018-03-03 08:25

本文选题：气管　切入点：组织工程　出处：《第四军医大学》2005年硕士论文　论文类型：学位论文

【摘要】：目的:气管疾病由于其解剖生理特点,直接危机患者的生命。外科治疗是解决气管疾病的首选方法,但对于切除范围长度在6cm以上的病变,直接端端吻合成功机率很小。因此,寻求一种理想的气管替代物是解决这一问题的关键。利用人工材料、自体组织和气管移植等方法重建气管,术后都存在着一定程度的狭窄,严重影响患者的生活质量。近年来组织工程学的兴起及其突飞猛进的发展,可望在这一领域做出突破性的贡献。依据我们长期以来在气管重建领域的研究,对气管重建后软骨细胞的再生,气管粘膜上皮的培养及气管重建后的再血管化等方面,已经积累了大量的实验基础。本实验以骨髓间质干细胞(mesenchymal stem cells,MSCs)为种子细胞,利用骨形态发生蛋白-2(bone morphogenetic protein,BMP-2)诱导MSCs定向分化为软骨细胞,复合聚乙醇酸(poly glycolic acid,PGA)支架材料上塑形,形成软骨组织。通过本实验的进一步研究,为构建组织工程气管软骨及气管粘膜上皮的复合提供理论和实验基础,最终构建组织工程气管,从结构和功能上完整重建气管,克服气管重建后软骨吸收造成的软化性狭窄,以及缺乏上皮覆盖导致的瘢痕性狭窄。方法:从兔骨髓中分离出MSCs,进行体外培养增殖。在培养基中加入BMP-2,诱导MSCs向软骨细胞分化,观察其形态学改变及碱性磷酸酶(alkaline phosphatase,ALP)活性,并进行ALP免疫组化染色观察。以不同浓度BMP-2
[Abstract]:Objective: because of its anatomical and physiological characteristics, trachea diseases directly threaten the life of patients. Surgical treatment is the first choice to solve trachea diseases, but the success rate of direct end-to-end anastomosis is very small for lesions with a resection range of more than 6 cm. To find an ideal tracheal substitute is the key to solve this problem. Using artificial materials, autologous tissue and trachea transplantation, there is a certain degree of stenosis after operation. The rise of tissue engineering and its rapid development in recent years are expected to make a breakthrough contribution in this field, according to our long-term research in the field of trachea reconstruction. A large number of experimental bases have been accumulated for the regeneration of chondrocytes after trachea reconstruction, the culture of tracheal mucosa epithelium and the vascularization after trachea reconstruction. In this experiment, mesenchymal stem cells (MSCs) were used as seed cells. Bone morphogenetic protein (BMP) -2bone morphogenetic protein (BMP-2) was used to induce MSCs to differentiate into chondrocytes and to form cartilage tissue on the scaffold material of poly glycolic acidine. It provides a theoretical and experimental basis for the construction of tracheal cartilage and epithelium in tissue engineering. Finally, the trachea is constructed. The trachea can be reconstructed completely in terms of structure and function, and the softening stenosis caused by cartilage absorption after trachea reconstruction can be overcome. And scarring stenosis due to a lack of epithelial coverage. Methods: MSCs were isolated from rabbit bone marrow and cultured in vitro. BMP-2 was added to the culture medium to induce the differentiation of MSCs into chondrocytes, and the morphological changes and alkaline phosphatase (ALP) activity were observed. The immunohistochemical staining of ALP was carried out. BMP-2 with different concentrations was observed.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R329.2

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