两种基因型蛔虫在小鼠体内的移行和分布

发布时间：2018-03-21 08:19

本文选题：蛔虫　切入点：基因型　出处：《南昌大学》2007年硕士论文　论文类型：学位论文

【摘要】： 似蚓蛔线虫即俗称人蛔虫(Ascaris lumbricoides Linnaeus，1758)是世界上流行地域最广、感染人数最多的病原生物之一，严重危害人类健康；而猪蛔虫(Ascaris suum Goeze，1782)则是造成生猪感染的最常见寄生虫，可造成巨大的经济损失。长期以来这两者的分类学地位一直存在争议。近几年，DNA技术应用于蛔虫种群的遗传研究，获得了一些新的发现。先前的研究表明，来自人体和猪体的蛔虫存在着不同的基因型，并且不同基因型的蛔虫在小鼠和猪体内表现出宿主寄生特异性差异，但这种差异是否和幼虫在宿主体内移行和分布有关，尚不清楚。本文对此进行了研究．本研究在实验条件控制下，用不同基因型(G1型和G3型)蛔虫感染期卵经食道感染C57BL／6雄性小鼠160只(感染剂量为1000个虫卵／每鼠)在感染早期(即感染后2h、4h、6h、8h、10h和12h)和中后期(即感染后1d、3d、5d、7d、9d、11d、15d、20d、25d和34d)的每个时间段各组解剖5只小鼠，检测小肠和大肠的虫卵和幼虫数量，以及血液，胸腔液，腹腔液，肝脏和肺部的幼虫数量，比较其在小鼠体内的移行和分布是否存在差异。结果显示，G1型虫卵和G3型虫卵在小鼠肠道孵化存在数量和部位差异。G1型孵化的幼虫数明显多于G3型幼虫，提示小鼠肠道环境对于G3型虫卵孵化可能不很适宜；G1型虫卵的孵化部位为小肠和大肠，而G3型孵化部位主要在大肠。两种基因型幼虫在小鼠脏器移行途径基本相同，但最早到达各脏器和消失时间存在差异。两种基因型幼虫在小鼠脏器的分布有所差异，G1型幼虫在各脏器的检出数明显多于G3型幼虫。感染后脏器平均重量观察结果表明，G1型蛔虫对于宿主脾脏重量的影响和损害明显大于G3型虫卵感染组，提示两种基因型蛔虫引起的宿主免疫反应可能存在差异。
[Abstract]:Ascaris lumbricoides Linnaeusus 1758) is one of the most prevalent and most infected pathogens in the world, seriously endangering human health, while Ascaris suum Goezeus 1782) is the most common parasite causing infection in live pigs. The taxonomic status of the two has long been in dispute. In recent years, DNA technology has been applied to genetic studies of Ascaris lumbricoides populations, and some new findings have been made. There are different genotypes of Ascaris lumbricoides from human body and pig body, and different genotypes of Ascaris lumbricoides show host parasitic specificity in mice and pigs, but this difference is related to the migration and distribution of larvae in the host body. It is not clear yet. This article has carried on the research to this. In this study, under the control of experimental conditions, C57BL / 6 male mice infected with different genotypes of Ascaris lumbricoides G1 and G3 were infected with oocytes of C57BL / 6 (1 000 eggs / mouse) at the early stage of infection (that is, 2 hours after infection, 4 h, 6 h, 8 h, 10 h and 12 h) and at the middle and late stages (1 d, 3 d, 5 d, 7 d, 9 d, 11 d, 15 d, 20 d, 20 d, 34 d), respectively. Five mice were dissected in each time period. The number of eggs and larvae in small intestine and large intestine, and the number of larvae in blood, pleural effusion, peritoneal fluid, liver and lung were determined to compare their migration and distribution in mice. The results showed that there were significant differences in the number and position of hatching between G 1 and G 3 eggs in the intestine of mice. The number of larvae hatched in G 1 type was significantly higher than that in G 3 type larvae. The results suggest that the intestinal environment of mice may not be suitable for the hatching of G3 type eggs. The hatching sites of G3 type eggs are small intestine and large intestine, while the location of G3 type eggs is mainly in large intestine. There were differences in the distribution of the two genotypic larvae in the viscera of mice. The detectable number of G1 larvae in each organ was significantly higher than that of G3 larvae. The average weight of organs was observed after infection. The results showed that the effect and damage of type G 1 roundworm on the weight of the host spleen was significantly greater than that of the group infected with G3 type eggs. These results suggest that the host immune response induced by two genotypes Ascaris lumbricoides may be different.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R382

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