骨髓基质细胞向血管壁细胞的分化

发布时间：2018-03-29 17:48

本文选题：骨髓基质细胞　切入点：间充质干细胞　出处：《第二军医大学》2006年硕士论文

【摘要】：种子细胞是组织工程研究的核心内容之一,理想的种子细胞应该具有容易获得、容易体外培养增殖、长期传代不改变生物学特征、抗原性小、组织修复能力强等特性。近年来发现骨髓基质细胞基本具备上述条件,能够分化为内皮样细胞和平滑肌样细胞。本文在体外分离培养出骨髓间充质干细胞,用生长因子、培养基和血清将其诱导分化为平滑肌细胞和内皮细胞;与血管内皮细胞联合培养也能导致骨髓间充质干细胞向平滑肌细胞分化,生理切应力能促进联合培养的骨髓间充质干细胞分化为更加成熟的平滑肌样细胞。本文还从骨髓中分离出CxCR4阳性细胞,证明了在这些细胞中存在PDFGR-β阳性的平滑肌祖细胞;从骨髓成功分离培养出了内皮祖细胞,研究了其分化和部分生物学特性。本研究为将这些骨髓来源的细胞作为种子细胞运用于心血管组织工程提供了依据。
[Abstract]:Seed cells are one of the core contents of tissue engineering research. The ideal seed cells should be easy to obtain, easy to culture and proliferate in vitro, the long-term passage does not change the biological characteristics, and the antigenicity is small. In recent years, bone marrow stromal cells have been found to be able to differentiate into endothelioid cells and smooth muscle like cells. Bone marrow mesenchymal stem cells were isolated and cultured in vitro. The culture medium and serum induced them to differentiate into smooth muscle cells and endothelial cells, and co-culture with vascular endothelial cells also resulted in the differentiation of bone marrow mesenchymal stem cells into smooth muscle cells. Physiological shear stress could promote the differentiation of bone marrow mesenchymal stem cells into more mature smooth muscle cells. We also isolated CxCR4 positive cells from bone marrow, which proved that PDFGR- 尾 positive smooth muscle progenitor cells existed in these cells. Endothelial progenitor cells (EPCs) were successfully isolated and cultured from bone marrow, and their differentiation and some biological characteristics were studied.
【学位授予单位】：第二军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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