RGS4与GR结构域相互作用的初步研究

发布时间：2018-04-30 06:46

本文选题：rgs4 + GR　；参考：《四川大学》2005年硕士论文

【摘要】：糖皮质激素的多种生理和药理效应可能与其非基因组效应有关，糖皮质激素受体(Glucocorticoid Receptor，GR)参与某些非基因组效应的信号转导，G蛋白信号转导调节因子(Regulator of G protein signaling 4，RGS4)与GR之间的相互作用可能是调节这一效应的重要途径之一。本研究拟采用酵母双杂交技术探讨RGS4与GR之间是否存在相互作用，进而明确相互作用的确切位点。该研究工作分为两个部分：1、利用酵母双杂交技术研究RGS4与GR各结构域之间的相互作用；2、利用GST-pulldown技术研究RGS4及GR各结构域蛋白之间相互作用。采用SD大鼠肝组织RNA，构建RGS4蛋白全编码框表达质粒作为诱饵蛋白表达质粒，并构建GR(AB、CD、EF、C、D)结构域的AD质粒，成功地建立了酵母双杂交体系。酵母双杂交结果显示，RGS4全编码框表达质粒转染酵母细胞后无自激活作用，GR各个
[Abstract]:Many physiological and pharmacological effects of glucocorticoids may be related to their non-genomic effects. Glucocorticoid receptor Glucocorticoid Receptorgrass is involved in the signal transduction of some non-genomic effects. The interaction between protein signaling 4 and gr may be one of the important ways to regulate this effect. In this study, yeast two-hybrid technique was used to investigate the interaction between RGS4 and gr, and the exact site of interaction was determined. The study was divided into two parts: 1. The interaction between RGS4 and gr domains was studied by yeast two-hybrid technique, and the interaction between RGS4 and gr domain proteins was studied by GST-pulldown technique. The full-coding frame expression plasmid of RGS4 protein was constructed as bait protein expression plasmid by RNAs from SD rat liver tissue, and the AD plasmid of GRABN CDSE-EFCU DG domain was constructed. The yeast two-hybrid system was successfully established. The results of yeast two-hybrid showed that the expression plasmid of RGS4 full coding frame had no self-activating effect on yeast cells after transfection.
【学位授予单位】：四川大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R341

【共引文献】