气阴两虚型2型糖尿病病证结合模型的实验研究

发布时间：2018-05-10 23:25

本文选题：气阴两虚 + 糖尿病　；参考：《辽宁中医药大学》2007年硕士论文

【摘要】： 目的：建立气阴两虚型2型糖尿病病证结合动物模型，，探讨气阴两虚型2型糖尿病病证结合动物模型的造模方法及成模标准。方法：将60只普通级Wister大鼠随机分为正常对照组、糖尿病空白对照组、气阴两虚模型组3组。气阴两虚模型组大鼠灌服中药青皮、附子，其余两组大鼠不做任何处理，连续4周，然后用中汇糖脉康对气阴两虚模型组大鼠进行反证。以大鼠的一般状态、血糖、血脂、皮质醇、甲状腺功能(FT_3)、胰岛素水平、血栓素B_2、6-酮前列腺素F_(1a)(6-Keto-PGF_(1a))水平以及胰腺、肾脏、肾上腺和甲状腺的病理作为观察指标。结果： 1．中药造模后气阴两虚模型组大鼠出现饮水增多、尿量增多、身体消瘦，精神萎糜，倦怠懒动，舌胖大，少津，光滑无苔等症状。 2．气阴两虚模型组及糖尿病空白对照组大鼠的血糖、血清甘油三酯(TG)、血清总胆固醇(TC)、低密度脂蛋白(LDL)、皮质醇、FT_3、胰岛素、血栓素B_2、6-酮前列腺素F_(1a)值与正常对照组比较均有明显差异；气阴两虚模型组大鼠的血清甘油三酯(TG)、血清总胆固醇(TC)、低密度脂蛋白(LDL)、FT_3、胰岛素、血栓素B_2值与糖尿病空白对照组比较均有显著差异。气阴两虚模型组及糖尿病空白对照组大鼠胰腺、肾脏、肾上腺和甲状腺的病理与正常对照组间有显著改变，而气阴两虚模型组与糖尿病空白对照组间无显著改变。 3、经反证后：气阴两虚模型组大鼠TG、TC、LDL、胰岛素、血栓素B_2的水平较反证前有所恢复且有显著性差异。结论：在传统的糖尿病造模方法的基础上，采用灌服不同性味中药的方法，以建立与临床证候相吻合的气阴两虚型2型糖尿病病证结合模型是切实可行的。前言随着人们生活水平的提高，糖尿病(DM)的发病率和患病率也日益升高，它已经成为继心脑血管病、肿瘤之后影响人类健康的第三大疾病，所以寻求切实可行的治疗方法已成为摆在众多医学工作者面前的一项紧迫的任务。祖国医学对糖尿病(消渴)的认识源远流长，在防
[Abstract]:Objective: to establish a combined animal model of type 2 diabetes mellitus with deficiency of qi and yin, and to explore the method and standard of making model of type 2 diabetes mellitus combined with disease and syndrome of deficiency of qi and yin. Methods: 60 normal Wister rats were randomly divided into 3 groups: normal control group, diabetes blank control group, Qi and yin deficiency model group. The rats of Qi-Yin deficiency model group were given Qingpi and aconite, the other two groups did not do any treatment for 4 weeks, then Zhonghui Tangmai Kang was used to reverse the syndrome of Qi and Yin deficiency model group. The general state, blood glucose, blood lipid, cortisol, thyroid function FT _ 3s, insulin level, thromboxane B _ 2oprostaglandin F _ (1) -keto-PGF _ (PGF) _ (1a), and the pathology of pancreas, kidney, adrenal gland and thyroid gland were taken as the indexes of observing the rats' general state, blood glucose, blood lipid, cortisol and thyroid function. Results: 1. The rats of Qi-Yin deficiency model group had symptoms such as drinking water increasing, urine quantity increasing, body wasting, mental wilting, languid and lazy, big tongue, little Tianjin, smooth and no fur, and so on. 2. There were significant differences in blood glucose, serum triglyceride TGN, serum total cholesterol TCU, low density lipoprotein (LDLN), cortisol FT-3, insulin, thromboxane B2O2-ketoprostaglandin Fasta 1a (compared with normal control group) in the model group of deficiency of qi and yin and the blank control group of diabetes mellitus. There were significant differences in serum triglyceride (TGG), serum total cholesterol (TC), low density lipoprotein (LDLX) FT _ 3, insulin, thromboxane B _ 2 between the Qi and Yin deficiency model group and the control group. The pathological changes of pancreas, kidney, adrenal gland and thyroid gland in the model group of deficiency of qi and yin and the control group of diabetes mellitus were significantly different from those in the normal control group, but there was no significant change between the model group of deficiency of qi and yin and the control group of diabetes mellitus. 3, after reverse identification, the levels of TGN TCL, insulin, thromboxane B2 in the model group of deficiency of both qi and yin recovered and had significant difference compared with those before the reverse. Conclusion: On the basis of the traditional diabetic model making method, it is feasible to establish a combined model of type 2 diabetes mellitus with Qi and Yin deficiency syndrome by taking different kinds of Chinese medicine with different sexual flavor. Preface With the improvement of people's living standard, the morbidity and morbidity of DMN (Diabetes Mellitus) are increasing day by day. It has become the third major disease that affects human health after cardio-cerebrovascular disease and tumor. Therefore, seeking practical treatment has become an urgent task for many medical workers. The understanding of diabetes mellitus (quench thirst) in traditional Chinese medicine has a long history.
【学位授予单位】：辽宁中医药大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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