甲状腺素对肠缺血—再灌注肠黏膜屏障功能的保护作用

发布时间：2018-06-15 07:12

本文选题：甲状腺素 + 缺血-再灌注　；参考：《延边大学》2005年硕士论文

【摘要】：多器官功能障碍综合征是目前临床中严重创伤和感染常见的并发症,其死亡率较高。目前研究认识到肠道细菌移位是产生细胞因子、炎症介子的一个重要因素,继而导致多器官功能障碍综合征与高代谢状态,而细菌移位是肠黏膜屏障功能受损的后果。肠黏膜受到破坏,肠道细菌移位,很快导致肠源性细菌感染,细菌内毒素则会激活细胞因子的释放,诱导瀑布效应的产生,导致脏器损害,甚至危及患者的生命,故保护肠黏膜屏障功能具有重要的意义。甲状腺素是机体重要的内分泌激素之一,其不仅对于维持心、脑等脏器的功能具有重要意义,而且近年研究还发现其对于肺、肝等均具有较明显的保护作用。本实验目的在于探讨肠缺血—再灌注24h时甲状腺素的代谢异常和肠黏膜屏障破坏之间的关系,阐明补充外源性甲状腺素对肠黏膜屏障功能保护的作用机制,为临床防治多器官功能障碍综合症提供必要的理论基础。方法:将Wistar大鼠39只随机分为4组,即假手术组(S,n=12)、肠缺血—再灌注组(G,n=8)、肠缺血—再灌注+生理盐水组(N,n=9)、肠缺血—再灌注+甲状腺素组(T,n=10)。利用肠系膜上动脉央闭法制作大鼠肠缺血—再灌注模型,并补充外源性甲状腺素。24h后测定外周血游离甲状腺素、促甲状腺素、磷酸肌酸激酶和门静脉血内毒素水平,同时做肠黏膜形态学检查。结果:再灌注24h后,G组和N组的血清甲状腺素水平明显低于S组,两者之间有
[Abstract]:Multiple organ dysfunction syndrome is a common complication of severe trauma and infection. At present, it has been recognized that intestinal bacterial translocation is an important factor in the production of cytokines and inflammatory mesons, which leads to multiple organ dysfunction syndrome and hypermetabolic state, and bacterial translocation is the consequence of impaired intestinal mucosal barrier function. Intestinal mucous membrane is destroyed, intestinal bacteria are translocated, intestinal bacterial infection is caused quickly, bacterial endotoxin activates the release of cytokines, induces waterfall effect, causes organ damage, and even endangers the life of patients. It is important to protect intestinal mucosal barrier function. Thyroxine is one of the most important endocrine hormones in the body. It not only plays an important role in maintaining the function of organs such as heart and brain, but also has obvious protective effects on lung and liver. The purpose of this study was to investigate the relationship between abnormal thyroid hormone metabolism and intestinal mucosal barrier damage at 24 h after intestinal ischemia-reperfusion, and to elucidate the mechanism of protective effect of exogenous thyroxine on intestinal mucosal barrier function. To provide the necessary theoretical basis for clinical prevention and treatment of multiple organ dysfunction syndrome. Methods: Thirty-nine Wistar rats were randomly divided into 4 groups: sham operation group (n = 12), intestinal ischemia / reperfusion group (n = 8), intestinal ischemia / reperfusion normal saline group (n = 9), intestinal ischemia / reperfusion thyroxine group (n = 10). The rat model of intestinal ischemia-reperfusion was established by the method of superior mesenteric artery closure. The levels of free thyroxine, thyrotropin, creatine phosphokinase and endotoxin in portal vein blood were determined 24 hours after supplementation of exogenous thyroxine. At the same time, the morphology of intestinal mucosa was examined. Results: the serum thyroxine levels in group G and group N were significantly lower than those in group S after 24 hours of reperfusion.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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