甲肝灭活疫苗（Vero细胞）毒株稳定性研究

发布时间：2018-06-16 19:34

本文选题：灭活疫苗 + 甲肝病毒　；参考：《吉林大学》2006年硕士论文

【摘要】：我国是肝炎大国,人群的甲肝感染率很高,属甲肝流行的高发区。预防甲肝最安全和有效的措施是接种甲肝疫苗。当前使用的疫苗共有2种类型:甲肝减毒疫苗和甲肝灭活疫苗。国内普遍使用甲肝减毒疫苗,而国外使用的全部为灭活疫苗。本课题是将甲肝患者粪便中分离的甲型肝炎病毒,在Vero细胞中进行适应性培养,选肓出高滴度的适应株,用于甲肝灭活疫苗研究。用选肓出的甲肝病毒毒株在Vero细胞中连续传代,检测其抗原滴度和感染性滴度,满意后可用此做毒种生产甲肝灭活疫苗。本实验选用的BL-08株,经各项理化实验证实为甲型肝炎病毒,该病毒经Vero细胞9次适应性传代以后,病毒滴度高达2048,感染性滴度为7.33 LogCCID50/ml,初步证明可做为甲型肝炎灭活疫苗的候选毒株.
[Abstract]:Our country is a big country of hepatitis, the infection rate of hepatitis A is very high, belong to the high incidence area of hepatitis A. The safest and most effective way to prevent hepatitis A is to vaccinate against it. There are two types of vaccines currently in use: attenuated hepatitis A vaccine and inactivated hepatitis A vaccine. Hepatitis A attenuated vaccine is widely used in China, while inactivated vaccine is used abroad. In this study, hepatitis A virus isolated from feces of patients with hepatitis A was cultured adaptively in Vero cells, and high titer adaptive strains were selected for the study of inactivated hepatitis A vaccine. The antigenic titer and infectious titer of the selected HAV strain were continuously subcultured in Vero cells and the inactivated hepatitis A vaccine could be produced by using this vaccine. The selected BL-08 strain was confirmed to be hepatitis A virus by various physical and chemical experiments. The virus was subcultured by Vero cells for 9 times. The titer of the virus was as high as 2048 and the infectious titer was 7.33 LogCCID 50 / ml, which was proved to be a candidate for inactivated hepatitis A vaccine.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

【共引文献】