白念珠菌生物被膜形成相关基因的功能研究

发布时间：2018-06-17 09:31

本文选题：白念珠菌 + 生物被膜　；参考：《第二军医大学》2005年博士论文

【摘要】：生物被膜形成是近年来发现的白念珠菌耐药新机制。本课题首先利用激光共聚焦显微镜确证Farnesol对白念珠菌生物被膜形成的抑制作用,随后采用cDNA芯片杂交法获得经Farnesol处理与未经处理生物被膜的差异表达基因,并以水—烃两相分离实验证明细胞表面疏水性下降是生物被膜形成受抑制的机制之一。将上述差异表达基因与其它白念珠菌生物被膜形成及耐药相关芯片杂交实验得到的差异表达基因作比较,并经克隆的全长测序及生物信息学分析,得到一全长基因THI13。采用“Ura-Blaster”技术敲除白念珠菌中的THI13基因,并进行表型分析。结果表明,THI13基因缺失可导致白念珠菌条件性的菌丝形成抑制以及对小鼠毒力的显著降低。
[Abstract]:Biofilm formation is a new mechanism of drug resistance found in Candida albicans in recent years. In this study, the inhibitory effect of Farnesol on biofilm formation of Candida albicans was confirmed by laser confocal microscopy, and then the differentially expressed genes were obtained by cDNA chip hybridization between treated and untreated biofilms. The hydrophobicity of cell surface was proved to be one of the mechanisms of inhibition of biofilm formation. The differentially expressed genes were compared with the differentially expressed genes obtained from other Candida albicans biofilm formation and drug-resistance-related microarray hybridization, and a full-length gene THI13was obtained by sequencing and bioinformatics analysis. THI13 gene from Candida albicans was knocked out by Ura-Blaster technique and phenotypic analysis was carried out. The results showed that the deletion of THI13 gene could result in the inhibition of conditioned mycelium formation and the decrease of toxicity to mice in Candida albicans.
【学位授予单位】：第二军医大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R379

【引证文献】