流感病毒壳聚糖微球鼻腔免疫疫苗的研究

发布时间：2018-06-17 10:31

本文选题：流感疫苗 + 壳聚糖　；参考：《吉林大学》2007年硕士论文

【摘要】： 目前预防流行感冒的主要手段是注射流感疫苗,基于目前对鼻腔免疫和新药载体的研究,为流感病毒壳聚糖微球鼻腔免疫疫苗的研究提供了可能,用壳聚糖包封流感病毒,通化鼻腔给药,达到免疫目的,避免注射痛触,提高生物利用度。研究发现选择TPP终浓度为400ug/ml,固化比为1:5,pH为5.6作为壳聚糖/流感病毒微球的制备条件,可制成粒径小于10um的微球。还进一步对壳聚糖/流感全病毒微粒鼻腔免疫进行初步研究,选6-8周的昆明鼠18只分三组,分别为壳聚糖/流感病毒样品组,流感病毒组,空白微球组,所有小鼠免疫3周后加强免疫一次。第一次免疫后第3周、第6周、第9周、12周采集血液和鼻道冲洗液。用间接ELISA方法检测血清中IgG和鼻道冲洗液中的IgA。实验结果表明壳聚糖/流感病毒能够在远端粘膜位点分泌较强的IgA,这对于通过呼吸道感染的流感病毒的预防十分重要,尽管其有效性还需要大量的实验证明,本论文说明了壳聚糖微球作为流感鼻腔免疫的可行性。
[Abstract]:At present, the main means of preventing influenza is to inject influenza vaccine. Based on the current research on nasal immunization and new drug carriers, it is possible to study the vaccine against influenza virus by chitosan microspheres. Tonghua nasal administration to achieve the purpose of immunity, avoid injection of pain, improve bioavailability. It was found that when the final concentration of TPP was 400ugml and the curing ratio was 1: 5g / ml, pH 5.6 was used as the preparation condition of chitosan / influenza virus microspheres, the microspheres with diameter smaller than 10um could be prepared. A preliminary study on nasal immunization of chitosan / influenza virus particles was carried out. Eighteen Kunming mice aged 6-8 weeks were divided into three groups: chitosan / influenza virus sample group, influenza virus group, and blank microsphere group. All mice were immunized once after 3 weeks. Blood and nasal irrigation fluid were collected 3 weeks, 6 weeks, 9 weeks and 12 weeks after the first immunization. IgG in serum and IgA in nasal irrigation fluid were detected by indirect Elisa. The results show that chitosan / influenza virus can secrete strong IgA at the distal mucosal site, which is very important for the prevention of influenza virus infected by respiratory tract, although its effectiveness needs a lot of experimental proof. In this paper, the feasibility of chitosan microspheres as nasal immunity for influenza was demonstrated.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R392

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