心理应激性勃起功能障碍大鼠模型建立及中医药干预研究

发布时间：2018-06-17 11:35

本文选题：心理应激 + 大鼠模型　；参考：《中国中医科学院》2006年硕士论文

【摘要】：目的建立多因素、长期、慢性心理应激性勃起功能障碍大鼠模型,并尝试用中药逍遥散对其进行干预治疗。方法 Wistar雄性大鼠50只,体重250—300g/只,随机平均分为5组,分别标记为空白组、模型组、疏肝组、电击组、单独饲养组;超大鼠5只,体重500—600g/只,标记为超重组。单独饲养组和超重组大鼠单笼饲养(1只/笼),空白组、模型组、疏肝组、电击组大鼠群养(5只大鼠/笼)。先对所有的实验大鼠进行糖水摄取实验,然后随机对模型组大鼠和疏肝组大鼠进行社交失败、Communication Box、行为限制、新环境拥挤4种刺激,同时给疏肝组大鼠用逍遥散水煎剂灌胃,空白组和模型组大鼠用饮用水灌胃。应激开始后的每周末对空白组大鼠进行阴茎勃起实验,对模型组和疏肝组大鼠进行性行为焦虑实验,同时观察记录大鼠阴茎勃起次数、呵欠次数、舔阴茎睾丸次数等数据。应激一个月后取阴茎、睾丸、血清并用酶法测定阴茎NO含量、ELISA测定血清睾酮含量。结果模型组大鼠阴茎勃起次数明显低于空白组,模型组大鼠阴茎NO,模型组大鼠睾丸指数明显高于空白组;疏肝组大鼠的各项指标与模型组相比有明显改善。结论本实验建立了多因素、长期、慢性心理应激下的ED模型;心理性应激造成ED的机理:长期慢性心理性应激可能通过使HPAT轴功能紊乱影响血清睾酮水平,进而使阴茎NO-cGMP信号通路紊乱,从而形成了ED;中药通过调节HPAT轴来发挥其调节全身各个组织器官的作用,间接对ED产生影响。
[Abstract]:Objective to establish a rat model of multiple factors chronic psychological stress erectile dysfunction and try to treat it with Xiaoyao Powder. Methods Fifty male 250-300g/ rats were randomly divided into 5 groups: blank group, model group, soothing liver group, electric shock group and 500-600g/ group. Single feeding group and superrecombinant rats were fed with single cage for 1 / cages, blank group, model group, soothing liver group and electric shock group for 5 rats / cage. First, all the experimental rats were tested on the intake of sugar water, and then the rats in the model group and the Shugan group were randomly given four kinds of stimuli of social failure, behavior limitation, new environment crowding, and the rats in the Shugan group were given the decoction of Xiaoyao Powder by stomach at the same time. Rats in blank group and model group were fed with drinking water. Every weekend after the stress, the rats in the blank group were tested for penile erection, and the rats in the model group and the soothing liver group were tested for sexual behavior anxiety. Meanwhile, the data of erectile number, yawning time and number of licking the testis of the penis were recorded and recorded at the same time. After one month of stress, the penis and testis were taken and the serum was determined by enzyme method. The serum testosterone content was determined by Elisa and enzyme-linked immunosorbent assay (Elisa). Results the number of penile erections in the model group was significantly lower than that in the blank group, and the testicular index of the model group was significantly higher than that of the blank group, and the indexes of the model group were significantly improved compared with the model group. Conclusion the model of Ed under long-term and chronic psychological stress was established, and the mechanism of Ed induced by psychological stress: Long-term chronic psychological stress may affect serum testosterone level by causing dysfunction of HPAT axis. Thus, the no-cGMP signaling pathway of the penis is disturbed and ED.The Chinese medicine exerts the function of regulating the organs and tissues of the whole body by regulating the HPAT axis, which has an indirect effect on Ed.
【学位授予单位】：中国中医科学院
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332;R277.52

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