钩端螺旋体OmpL1的表达及脑膜炎大肠杆菌IbeA单克隆抗体的制备

发布时间：2018-06-17 14:27

本文选题：钩端 + 螺旋体　；参考：《第一军医大学》2006年硕士论文

【摘要】：一、研究背景和目的问号钩端螺旋体(Leptospira interrogans)感染引起的钩端螺旋体病(Leptospirosis)，简称钩体病，是世界范围流行的自然疫源性的人兽共患病。由于其发病急、临床症状复杂多样且不典型，极易误诊和漏诊。所以，钩体病的实验室诊断和预防对于控制钩体病的流行具有重要的意义。Haake等1993年首次从流感伤寒群钩体基因组DNA中鉴定了一个全长963bp，编码320个氨基酸的穿膜蛋白OmpL1。用Southern杂交证实ompL1基因存在于多群问号(致病性)钩体的基因组DNA中，在非致病性钩体中检测不到，说明OmpL1是致病性钩体所特有。本文第一部分工作是建立以问号钩端螺旋体致病蛋白OmpL1为诊断抗原的检测OmpL1抗体的免疫层析技术。而黄疸出血型赖株(56601)钩体是我国独有的菌型并且具有代表性，，流行范围广，毒力强。因此本实验选用我国优势流行血清群黄疽出血群赖株ompL1基因作为靶基因，采用基因工程技术获得体外高效表达的重组问号钩端螺旋体黄疽出血群赖株融合蛋白OmpL1。细菌性脑膜炎是中枢神经系统(central nerve system，CNS)最常见最严重的感染性疾病。而脑膜炎大肠杆菌(Escherichia coli，E.coli)是引起新生儿脑膜炎的主要病原体。在脑膜炎大肠杆菌的致病机理中，细菌穿过血脑屏障是致病的关键环节，但其机制尚不清楚。人的脑微血管内皮细胞(brain microvascula endothelial cell，BMEC)是构成血脑屏障(blood-brain barrier,BBB)的重要成分
[Abstract]:Background and objective Leptospira interroganses infection caused by Leptospira interroganses infection in Leptospirosis japonica, Is a worldwide epidemic of natural epidemic zoonosis. Because of its acute onset and complicated and atypical clinical symptoms, it is prone to misdiagnosis and missed diagnosis. Therefore, the laboratory diagnosis and prevention of leptospirosis are of great significance in controlling the prevalence of leptospirosis. Haake et al first identified a full-length protein OmpL1 encoding 320 amino acids from genomic DNA of influenza typhoid group Leptospira in 1993. It was confirmed by Southern blotting that ompL1 gene existed in genomic DNA of multiple group question mark (pathogenic) leptospirosis, but could not be detected in non-pathogenic Leptospira, suggesting that OmpL1 is unique to pathogenic Leptospira. The first part of this paper is to establish an immunochromatographic technique for the detection of OmpL1 antibodies using the Leptospira interrogans pathogenic protein OmpL1 as diagnostic antigen. Leptospira is unique and representative in China with wide epidemic range and strong virulence. Therefore, ompL1 gene was selected as the target gene of Chinese dominant serogroup yellow gangrene haemorrhage colony, and the recombinant Leptospira yellow haemorrhage colony fusion protein OmpL1 was obtained by gene engineering technique in vitro. Bacterial meningitis is the most common and severe infectious disease in central nervous system. Escherichia coli E. coli is the main pathogen of neonatal meningitis. In the pathogenic mechanism of meningitis Escherichia coli, bacteria crossing the blood-brain barrier is the key link, but its mechanism is not clear. Human brain microvascula endothelial cells (BMECs) are important components of blood-brain barrier (BBB).
【学位授予单位】：第一军医大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R377

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