肝源磷脂酰胆碱对四氯化碳肝细胞损伤的保护作用

发布时间：2018-06-21 03:33

本文选题：肝源磷脂酰胆碱 + CC14肝损伤　；参考：《辽宁师范大学》2006年硕士论文

【摘要】：磷脂酰胆碱(Phosphatidylcholine，PC)也称卵磷脂。它是细胞膜的重要组成成分，起保护层的作用，能重新修复由于自由基攻击生物大分子而产生的膜损伤；它具有胆碱成分，胆碱对脂肪具有亲和力，可使脂肪以磷脂形式由肝脏通过血液输送出去，从而防止脂肪肝的形成；它是提供神经物质乙酰胆碱的前体物质，具有健脑功能；还用于脂质体的研究，以及作为抗癌药物的研究，在食品、医药、化妆品等领域有广阔的应用前景。肝脏是人体代谢的中心站，含有极其丰富的PC。在哺乳动物肝脏中，除了CDP-胆碱通路(又称Kennedy通路)外，还存在一条特有的PC合成通路，即磷脂酰乙醇胺N-甲基转移酶(phosphatidylethanolamine N-methyltransferase，PEMT)催化磷脂酰乙醇胺(phosphorylethanolamine，PE)甲基化生成PC的通路。研究发现，肝病时合成PC的PEMT活性降低。我们实验室利用基因转染技术过表达PEMT2后，发现大鼠肝癌细胞的生长被显著抑制，并诱发其细胞凋亡。提示，肝脏中的PC可能具有独特的作用。有研究表明，肝细胞损伤时，细胞膜的PC代谢和含量会发生明显的变化。那么，补充外源肝性的PC是否有利于受损伤的肝细胞恢复或减轻肝损伤的程度? CCl_4是目前建立肝细胞实验性损伤模型的常用药物，能成功的复制肝损伤模型。CCl_4可通过P-450酶系统脱卤素后形成CCl_3自由基，后者能从不饱和脂肪酸分子中夺取氢离子，引起脂质过氧化。本研究以CCl_4诱导正常人肝细胞HL7702损伤，复制肝细胞损伤模型，，观察了补充肝源PC对肝
[Abstract]:Phosphatidylcholine Phosphatidylcholine (PCP) is also known as lecithin. It is an important component of the cell membrane and acts as a protective layer that restores membrane damage caused by free radicals attacking biomolecules; it has a choline component, and choline has affinity for fat. Fat is transported through the blood by the liver in the form of phospholipid, thus preventing the formation of fatty liver; it is a precursor material to provide the nerve substance acetylcholine and has the function of strengthening the brain; it is also used in the study of liposome. As well as the research of anticancer drugs, it has broad application prospect in food, medicine, cosmetics and so on. The liver is the center of human metabolism, containing extremely rich PCS. In mammalian livers, in addition to the CDP- choline pathway (also known as Kennedy pathway), there is a unique PC synthesis pathway. Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the methylation of phosphatidylethanolamine to PC. It was found that the PEMT activity of PC was decreased during liver disease. After overexpression of PEMT2 by gene transfection technique in our laboratory, we found that the growth of rat hepatoma cells was significantly inhibited and apoptosis induced. These results suggest that PC may play a unique role in the liver. Some studies have shown that the metabolism and content of PC in the cell membrane change obviously when the liver cell is damaged. So, is supplementation of exogenous hepatic PC beneficial to the recovery of the injured hepatocytes or the reduction of the degree of liver injury? CClst4 is a common drug used to establish experimental liver injury model. CClS4 can successfully replicate the liver injury model. CClS4 can dehalogenate through P-450 enzyme system to form CCl3 free radical, which can capture hydrogen ion from fatty acid molecule. Cause lipid peroxidation. In this study, normal human hepatocytes HL7702 were induced by CClS4, and the model of hepatocyte injury was established. The effects of liver PC supplementation on liver were observed.
【学位授予单位】：辽宁师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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