Secretin对孤束核中胃肠相关神经元活动的影响的研究

发布时间：2018-06-21 04:49

本文选题：促胰泌素 + 孤束核　；参考：《重庆医科大学》2006年硕士论文

【摘要】： 目的研究中枢和外周的促胰泌素(secretin)对孤束核中胃肠相关神经元活动的影响及其机制。方法1.记录乌拉坦麻醉的大鼠孤束核中与电刺激膈下迷走神经相关的神经元自发和诱发放电。观察在外周静脉分别注射不同剂量的secretin(0.04、0.08、0.16μg·kg-1),了解外周外源性secretin对孤束核中与电刺激膈下迷走神经相关神经元的放电的作用。2.记录乌拉坦麻醉的大鼠孤束核中与扩胃相关的神经元的自发和诱发放电。观察在外周静脉分别注射不同剂量的secretin(0.04、0.08、0.16μg·kg-1)和兔促胰液素抗血清(secretin antiserum)对该类神经元放电的影响。 3.记录乌拉坦麻醉的大鼠孤束核中与扩胃相关的神经元的自发和诱发放电,观察在侧脑室注射微量secretin(0.1μg·ml secretin 1μl)后对该类细胞自发和诱发放电的影响。4.记录乌拉坦麻醉的大鼠孤束核中与电刺激膈下迷走神经相关的神经元自发和诱发放电,观察在侧脑室注射微量secretin(0.1μg·ml secretin 1μl)后对该类细胞自发和诱发放电的影响。5.用高效液相色谱法测定脑脊液中的谷氨酸(Glu)和γ-氨基丁酸(GABA)的含量,观察在静脉给予一定剂量secretin对脑脊液中Glu和GABA的含量的影响,以及给予不同剂量的secretin对反复扩胃后脑脊液中Glu和
[Abstract]:Aim to study the effects of central and peripheral secretin on gastrointestinal related neurons in nucleus tractus solitarii and its mechanism. Method 1. Spontaneous and evoked discharges of neurons associated with electrical stimulation of the subphrenic vagus nerve were recorded in the nucleus tractus solitarii of rats anesthetized with uratan. To observe the effect of exogenous secretin on the discharge of subphrenic vagus nerve related neurons in nucleus of solitary tract by injecting different doses of secretinus 0.04 (0.08 渭 g 路kg ~ (-1) into peripheral vein respectively, and to investigate the effect of exogenous secretin on the discharges of subphrenic vagus neurons in the nucleus tractus solitarii. Spontaneous and evoked discharges of neurons associated with gastric dilatation in the nucleus tractus solitarius were recorded. The effects of different doses of secretinine 0.04 (0.08 渭 g 路kg ~ (-1) and rabbit antiserum secretin antiserum3 on the discharge of these neurons were observed. The spontaneous and evoked discharges of neurons associated with gastric distending in the nucleus tractus solitarius were recorded and the effects of intraventricular injection of secretin(0.1 渭 g ml secretin 1 渭 l on the spontaneous and evoked discharges of these neurons were observed. The spontaneous and evoked discharges of neurons associated with electrical stimulation of subphrenic vagus nerve were recorded in the nucleus tractus solitarii of rats anesthetized by urartan. The effects of microinjection of secretin(0.1 渭 g ml secretin 1 渭 l into lateral ventricle on the spontaneous and evoked discharges of these neurons were observed. The content of glutamate Gluand 纬 -aminobutyrate in cerebrospinal fluid (CSF) was determined by high performance liquid chromatography (HPLC). The effects of intravenous administration of secretin on the contents of Glu and GABA in cerebrospinal fluid (CSF) were observed. The effects of different doses of secretin on the levels of Glu and Glu in cerebrospinal fluid (CSF) after repeated gastric dilatation
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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