新疆部分人群SEN病毒的感染状况及亚型分析

发布时间：2018-06-21 05:00

本文选题：SEN病毒 + 聚合酶链反应　；参考：《石河子大学》2007年硕士论文

【摘要】： 目的: 1.建立聚合酶链式反应(PCR)筛查SENV的反应体系和流程; 2.检测SENV-D和SENV-H在新疆地区部分人群中的流行情况; 3.对SENV-D和H亚型进行进化地位分析。方法: 1.对象:选取98例健康供血员作为对照组,113例HBV阳性、57例抗-HCV阳性、27例ALT升高者作为病例组; 2.用Ready PCR血清病毒DNA纯化系统提取样本血浆中的病毒DNA,针对SENV ORF1部分区域设计、合成引物,采用巢式聚合酶链反应(nested-PCR)扩增SENV-D和SENV-H亚型; 3.运用SPSS10.0进行数据分析;用DNASTAR软件分析SENV的系统进化情况。结果: 1.建立的巢式聚合酶链式反应(nPCR)具有较高的特异性和重复性; 2. SENV在对照组、HBV阳性组、抗-HCV阳性组、ALT升高组检出率分别为35.7%、51.3%、36.8%和14.8%,其中HBV阳性组的检出率与对照组有显著差异(P 0.05); 3. SENV在汉族、维吾尔族和回族中的检出率分别为41.9%、38.9%和15.8%; 4.系统进化树分析,SENV-D和H亚型在进化过程中无地域差别。结论: 1.建立了同时检测SEN病毒D和H型的巢式聚合酶链检测方法(nPCR); 2.获得了3个SENV D型片段和2个SENV H型片段; 3.在新疆汉族、维吾尔族和回族中都存在SENV D和H亚型感染; 4. SENV与HBV有相似的传播途径,但可能更多依赖非输血传播; 5.系统发育分析显示新疆分离株(SENV-D-XJ6、XJ-58和XJ-111以及SENV-H-X-J5和XJ-172)分别与己发表的SENV-D(AB059352)和SENV-H(AB059353)日本分离株同属一个亚型;新疆分离株属于TTV超家族。
[Abstract]:Objective: 1. To establish the reaction system and procedure for screening SENV by polymerase chain reaction (PCR). 2. To detect the prevalence of SENV-D and SENV-H in some populations in Xinjiang. The phylogenetic status of SENV-D and H subtypes was analyzed. Methods: 1. Participants: 98 healthy blood donors were selected as control group, 113 cases were HBV positive and 57 cases were anti-HCV positive and 27 cases were alt elevation. 2. Virus DNA was extracted from plasma by ready PCR system. Primers were synthesized and amplified by nested polymerase chain reaction (nested polymerase chain reaction) to amplify SENV-D and SENV-H subtypes. 3. SPSS 10.0 was used to analyze the data and DNASTAR software was used to analyze the evolution of SENV. Results: 1. The nested polymerase chain reaction (nPCR) has high specificity and reproducibility. 2. The positive rates of SENV in HBV positive group and anti-HCV positive group were 35.7% and 14.8%, respectively. There was significant difference between HBV positive group and control group (P 0.05; 3). The positive rates of SENV in Han, Uygur and Hui nationalities were 38.9% and 15.88%, respectively. Phylogenetic tree analysis showed that there was no difference between SENV-D and H subtypes in the evolution process. Conclusion: 1. A nested polymerase chain analysis method for simultaneous detection of SEN virus D and H was established. Three SENV D-type fragments and two SENV H-type fragments were obtained. SENV D and H subtypes were found in Han, Uygur and Hui nationalities in Xinjiang. SENV and HBV have similar transmission routes, but may rely more on non-transfusion transmission. Phylogenetic analysis showed that SENV-D-XJ6 XJ-58 and XJ-111, SENV-H-X-J5 and XJ-172) belong to the same subtype as the previously published SENV-DX AB059352) and SENV-HGX AB059353), and Xinjiang isolates belong to the TTV superfamily.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R373

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