骨髓间充质干细胞的成骨性诱导

发布时间：2018-06-22 07:33

本文选题：骨髓间充质干细胞 + 全骨髓贴壁法　；参考：《中华中医药学刊》2014年09期

【摘要】：目的:观察大鼠骨髓间充质干细胞向成骨细胞诱导分化的效果。方法:采用全骨髓贴壁法培养SD大鼠骨髓间充质干细胞,把生长状态良好的第3代骨髓间充质干细胞,分为两组,对照组用DMEM-LG培养基培养;实验组用含有成骨诱导剂的DMEM-LG培养基培养。结果:全骨髓贴壁法培养的原代骨髓间充质干细胞贴壁且呈梭形;经成骨诱导剂诱导后骨髓间充质干细胞呈卵圆形贴壁生长,碱性磷酸酶活性明显强于对照组(P0.01),茜素红染色出现阳性的钙化结节。结论:全骨髓贴壁法培养骨髓间充质干细胞方法简单、实用,所培养的骨髓间充质干细胞在成骨诱导后表现了成骨细胞的形态学和生物学特性。
[Abstract]:Aim: to observe the differentiation of rat bone marrow mesenchymal stem cells into osteoblasts. Methods: bone marrow mesenchymal stem cells of SD rats were cultured by whole bone marrow adherent method. The third generation of bone marrow mesenchymal stem cells were divided into two groups. The control group was cultured on DMEM-LG medium. The experimental group was cultured on DMEM-LG medium containing osteogenic inducer. Results: primary bone marrow mesenchymal stem cells cultured by whole bone marrow adherent method were fusiform and oval adherent to bone marrow mesenchymal stem cells induced by osteogenic inducer. Alkaline phosphatase activity was significantly higher than that of control group (P0.01). Alizarin red staining showed calcified nodules. Conclusion: bone marrow mesenchymal stem cells cultured by whole bone marrow adherent method is simple and practical. The cultured bone marrow mesenchymal stem cells exhibit the morphological and biological characteristics of osteoblasts after osteogenesis induction.
【作者单位】：山东中医药大学;山东省中医药研究院;山东省医学科学院附属医院;中国中医科学院望京医院;
【基金】：国家自然科学基金项目(81273776) 山东省科技攻关计划项目(2010GCSF10278) 山东省自主创新重大计划项目(2012ZHZX1C0405) 山东中医药科技发展计划(2009-128) 山东省科技发展专项(2012G0021846)
【分类号】：R329

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