一氧化氮及供体SNAP对细菌生长的影响的体外实验研究

发布时间：2018-06-23 05:41

  本文选题：一氧化氮 + 一氧化氮供体　； 参考：《中国医科大学》2005年硕士论文

【摘要】：目的 一氧化氮(NO)是近年发现的一个高活性气体信号分子,除舒张血管外还是参与炎症反应、感染性免疫调控活动与神经传导的重要介质。NO供体是能释放活性NO的化合物。研究发现NO具有明显抑制细菌内毒素引起的炎性反应过程。已知抗菌药物对细菌内毒素的清除及其诱发的炎性反应和组织损伤无效。如果重症肺炎在使用抗菌药物的同时,吸入NO气体或雾化吸入NO供体,可能收到抗菌、抗炎的双重效果。本研究目的在于探讨NO及其供体SNAP的抑菌作用。 实验一、不同浓度NO对五种病菌生长影响的体外实验 方法 将表皮葡萄球菌、大肠杆菌、金黄色葡萄球菌、绿脓杆菌、白色念珠菌五种常见呼吸道致病菌稀释为10~2CFU,按10μl/碟、50μl/碟,分别接种于MH琼脂培养皿。分为空气暴露组和10PPM、25PPM、40PPM NO暴露组。在温度37℃,湿度45-60%的细菌孵育箱,培养24小时。取出,进行CFU计数,并计算出平均抑菌率。 结果 1.10μl/碟、50μl/碟白色念珠菌暴露于NO浓度为10PPM、25PPM、40PPM中明显抑制(P0.05)。 2.10μl/碟表皮葡萄球菌暴露于NO浓度为10PPM、25PPM、40PPM中明显抑制(P0.05)。50μl/碟表皮葡萄球菌暴露于NO浓度为25PPM、
[Abstract]:Objective nitric oxide (no) is a highly active gas signaling molecule found in recent years. The important mediators of infectious immune regulation and nerve conduction. No donor is a compound that can release active no. It was found that no could inhibit the inflammatory reaction induced by bacterial endotoxin. Bacterial endotoxin clearance, inflammatory response and tissue damage induced by antimicrobial agents are not effective. If severe pneumonia inhale no gas or atomized no donor while using antimicrobial agents, it may have the dual effect of anti-bacterial and anti-inflammatory. The aim of this study was to investigate the bacteriostatic effect of no and its donor SNAP. Experiment 1. In vitro, Staphylococcus epidermidis was used to study the effects of different concentrations of no on the growth of five kinds of bacteria. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were diluted to 10 ~ 2 CFU. They were divided into air exposure group and 10 PPMN 25 PPMN 40 PPM no exposure group. The culture temperature was 37 鈩，

本文编号：2056037