失血性贫血小鼠骨髓间充质干细胞向成骨成脂分化的变化

发布时间：2018-06-23 07:41

本文选题：间充质干细胞 + 脂肪细胞　；参考：《中国实验血液学杂志》2014年01期

【摘要】：本研究旨在检测骨髓间充质干细胞(multipotent mesenchymal stem cells,MSC)向成骨、成脂分化的变化,探讨骨髓间充质干细胞的分化状态对骨髓造血功能的影响。先对实验小鼠每隔1-2 d行内眦静脉取血0.3 ml,4周构建贫血模型,通过检测贫血小鼠外周血常规指标、网织红细胞比例、骨髓细胞造血集落形成以验证小鼠模型。通过检测贫血模型小鼠成纤维细胞集落形成、骨髓细胞和脂肪细胞的数量,并用实时定量PCR的方法检测骨髓成骨、成脂分化相关基因的表达来研究骨髓MSC分化潜能的改变。结果显示,与对照组相比,贫血小鼠红细胞数量和血红蛋白水平明显下降,网织红细胞比例增高,骨髓细胞造血集落形成单位数量增多;骨髓造血细胞增多,脂肪细胞减少;骨髓成纤维细胞集落形成单位增多并显著向成骨细胞分化;骨髓成骨分化相关基因Runx2和OSX的表达明显升高,而成脂相关基因aP2、PPARγ2的表达明显降低。结论:在骨髓造血功能活跃期小鼠骨髓间充质干细胞向成骨细胞分化能力增强,向脂肪细胞分化能力减弱。
[Abstract]:The purpose of this study was to detect the changes of bone marrow mesenchymal stem cells (multipotent mesenchymal stem cells) into osteogenesis and adipogenic differentiation, and to investigate the effect of bone marrow mesenchymal stem cells differentiation on bone marrow hematopoietic function. The anemia model of experimental mice was established by taking blood from inner canthus vein every 1-2 days for 4 weeks. The normal indexes of peripheral blood, the proportion of reticulocyte and the formation of hematopoietic colony of bone marrow cells in anemia mice were detected to verify the model. The colony formation of fibroblasts, the number of bone marrow cells and adipocytes in anaemic model mice were detected. The bone marrow osteogenesis and the expression of genes associated with adipogenic differentiation were detected by real-time quantitative PCR to study the change of differentiation potential of bone marrow MSC. The results showed that compared with the control group, the erythrocyte count and hemoglobin level of anemia mice decreased significantly, the ratio of reticulocyte increased, the number of hematopoietic colony forming units of bone marrow cells increased, the hematopoietic cells of bone marrow increased, and the adipocytes decreased. The colony forming units of bone marrow fibroblasts increased and differentiated significantly into osteoblasts, while the expression of bone marrow osteoblast differentiation related genes Runx2 and OSX increased significantly, while the expression of adipogenic associated gene aP2nPPAR 纬 2 decreased significantly. Conclusion: the differentiation ability of bone marrow mesenchymal stem cells into osteoblasts was enhanced and adipocyte differentiation was weakened during the active period of bone marrow hematopoiesis.
【作者单位】：北京大学人民医院;北京积水潭医院;
【基金】：国家自然科学基金资助项目(81270572)
【分类号】：R329.28

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