不同时段深低温冷藏骨组织的超微结构与力学研究

发布时间：2018-06-23 07:50

本文选题：深低温冷藏 + 同种异体骨　；参考：《福建医科大学》2006年硕士论文

【摘要】： 目的:探讨不同时段深低温冷藏对骨组织结构与力学性能的影响,为临床同种异体骨移植提供基础理论。方法:研究材料为成人胫骨,在相同部位取其骨片、骨段样本,以15%DMSO为低温保护剂,采用两步冷冻法,即实验样本用-55℃的冷冻预处理,在-196℃液氮中保存2、4、6、8、10、12个月后,实验前对不同时段的标本用40℃水浴快速复温,行光镜与电镜观察、抗压与抗折的力学测试。结果:(1)骨的显微结构:在冷藏2、4个月组的骨细胞、哈氏系统的中央管接近对照组;冷藏6个月之后开始出现骨细胞减少,中央管扩大,有的呈囊样扩张;冷藏2、4、6、8个月组的骨基质保持正常,10个月组之后出现水肿、小片状凝固坏死、或玻璃样变性。(2)骨的超微结构:冷藏2、4个月组的骨细胞结构正常;冷藏6个月之后开始出现骨细胞固缩,冷藏2、4、6个月组的骨胶原纤维排列整齐,冷藏8个月组的骨胶原纤维排列模糊不清。(3)骨的力学测试:冷藏6个月组的强度极限、冷藏6个月组的弹性模量和冷藏10个月组的压缩率与对照组有统计学意义上的区别(P0.05),但各冷藏实验组的极限荷载与对照组没有统计学意义上的区别(P0.05)。结论:(1)骨组织深低温冷藏随着冷藏时间段的延长出现组织结构进行性损伤与力学性能减退;(2)深低温冷藏6个月是骨组织损伤的开始时段;(3)深低温冷藏对骨细胞与骨基质的损伤不在同一时段,呈先、后出现;(4)力学性能变化晚于骨组织结构的改变。
[Abstract]:Objective: To investigate the effects of deep cryopreservation on bone tissue structure and mechanical properties in different periods, so as to provide basic theory for clinical allograft.
Methods: To study the material for adult tibia, take the bone slices and bone samples in the same part, take 15%DMSO as the cryogenic protective agent, use the two step freezing method, that is, the experimental samples are frozen in -55 C for 2,4,6,8,10,12 months in the liquid nitrogen of -196 C, and the specimens of the different segments are rewarming quickly before the experiment, and the light and electron microscopic views are used. Inspection, mechanical testing of compression and bending.
Results: (1) the microstructure of the bone: the bone cells in the cold storage 2,4 month group, the central tube of the Hart's system was close to the control group. After 6 months of cold storage, the osteocyte decreased, the central canal expanded, and some cystic dilatation; the bone matrix in the cold storage group of 2,4,6,8 months remained normal, and the edema, small slice necrosis, or glass like after the 10 month group were found. (2) ultrastructure of bone: the bone cell structure in the cold storage 2,4 month group was normal; bone cell fixation began to appear after 6 months of cold storage, the bone gelatin fibrils in the cold storage group of 2,4,6 months were arranged orderly, and the collagen fibrils in the 8 month cold storage group were ambiguous. (3) the mechanical test of the bone: the strength limit of the 6 month cold storage group and the 6 month cold storage bomb The compression rate of the sex modulus and the 10 month cold storage group was significantly different from the control group (P0.05), but the limit load of the cold storage experimental groups was not statistically different from that of the control group (P0.05).
Conclusions: (1) the tissue structure progressive injury and mechanical properties decrease with the prolongation of cold storage time, and (2) deep cryogenic cold storage for 6 months is the beginning of bone tissue damage; (3) the damage of bone cells and bone matrix in deep cryogenic cold storage is not at the same time, and appears first and later; (4) changes of mechanical properties are later than those of bone group. The change of fabric structure.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

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