两歧双歧杆菌干预治疗鼠伤寒沙门菌感染小鼠的生物保护作用

发布时间：2018-06-23 08:23

本文选题：两岐双岐杆菌 + 鼠伤寒沙门菌　；参考：《新疆医科大学》2006年硕士论文

【摘要】：目的：以鼠伤寒沙门菌(Salmonella typhimurium，STM)感染小鼠为模型，研究两岐双岐杆菌(Bifidobacterim bifidum，B．bifidum)在体内对STM感染小鼠的治疗作用。方法：先用硫酸链霉素给正常小鼠灌胃2d，出现菌群失调症状，再用STM灌胃造成小鼠STM感染，使用两岐双岐杆菌液干预治疗STM感染小鼠，检测不同治疗时间各组小鼠粪便STM菌落数、肝脾指数、血及脾中TNF-a的含量。采用SPSS12.0统计软件包进行数据处理分析，统计学方法采用双因素重复测量资料的方差分析、单因素随机化设计的方差分析、卡方检验、多组均数比较的非参数秩和检验。结果：随着治疗天数的增加，，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较结果均为P＜0.01；组间比较，双歧杆菌组STM数下降最快。未干预组、生理盐水组及双歧杆菌组肝脾指数比较均P＞0.05；STM感染各组与正常组血及脾中TNF-a的含量比较均P＜0.05；STM感染各组中未干预组、生理盐水组及双歧杆菌组血中TNF-a的含量比较P＞0.05，脾中TNF-a的含量比较P＜0.05；组间比较，未干预组与双歧杆菌组比较，P＜0.05。结论：随着治疗天数的增加，STM菌落数逐渐减少，未干预组、生理盐水组及双歧杆菌组比较有统计学意义，提示双歧杆菌对小鼠STM感染有治疗作用；根据肝脾指数的比较结果，说明其对免疫器官干预治疗作用不够显著；正常组、未干预组、生理盐水组及双歧杆菌组血及脾TNF-a含量比较表明，STM感染各组血及脾中TNF-a含量均高于正常组，且双歧杆菌组最高，提示双歧杆菌干预对感染机体免疫细胞分泌TNF-a有一定促进作用。
[Abstract]:Objective: to study the therapeutic effect of Bifidobacterium bifidum (Bifidobacterium bifidum) on STM infected mice in vivo using Salmonella typhimurium STM as a model. Methods: the normal mice were given streptomycin sulfate for 2 days, and then STM was used to induce STM infection in mice. The STM infected mice were treated with bifidobacterium solution. The fecal STM colony number, liver and spleen index, TNF-a content in blood and spleen were measured. SPSS 12.0 software package was used to process the data, and the statistical method was used to analyze the variance of double-factor repeated measurement data, single factor randomization design analysis of variance, chi-square test, and non-parametric rank sum test of multi-group mean comparison. Results: with the increase of treatment days, the number of STM colonies gradually decreased. The results of no intervention group, normal saline group and Bifidobacterium group were all P < 0.01. The levels of TNF-a in blood and spleen of non-intervention group, normal saline group and bifidobacterium group were significantly higher than those of control group (P > 0.05). The contents of TNF-a in blood and spleen in normal saline group and bifidobacterium group were P > 0.05 and P < 0.05, respectively, and there were significant differences between non-intervention group and Bifidobacterium group (P < 0.05). Conclusion: with the increase of treatment days, the number of STM colonies gradually decreased, and the number of STM colonies in non-intervention group, normal saline group and bifidobacterium group were significantly higher than those in control group, suggesting that Bifidobacterium could treat STM infection in mice, according to the comparison of liver and spleen index. The results showed that TNF-a content in blood and spleen of normal group, non-intervention group, normal saline group and bifidobacterium group was higher than that of normal group, and the TNF-a content in blood and spleen of STM infected group was higher than that of normal group, and that of Bifidobacterium group was the highest. The results suggest that the intervention of Bifidobacterium can promote the secretion of TNF-a by immune cells.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R371

【引证文献】