松果体及褪黑素在大鼠胸腺T细胞分化发育中的作用

发布时间：2018-06-24 21:16

本文选题：大鼠 + 松果体　；参考：《福建医科大学》2005年博士论文

【摘要】：自从1926年Berman提出松果体与胸腺相关的概念以来,许多实验研究表明松果体与胸腺之间存在较明显的功能联系,然而迄今为止的研究绝大多数为体外实验,且研究手段较局限,因而松果体与胸腺结构和功能的关系及其相关机制仍然不明确。本研究运用大鼠松果体摘除动物模型,从以下四个方面探讨松果体及其褪黑素对大鼠胸腺T细胞分化发育的作用及其可能的机制。一、松果体及褪黑素对大鼠胸腺T细胞分化发育的影响有关松果体及其褪黑素对胸腺T细胞分化发育过程中不同亚群的影响及其可能的机制尚未见报道。本部分运用免疫细胞化学、计算机图像分析以及CD_4、CD_8双色直标单抗反应法流式细胞术检测松果体摘除及补充褪黑素后胸腺和血液T细胞亚群的比例,观察松果体摘除后胸腺和血液T细胞CD4/8不同亚群的变化,以及补充褪黑素的影响,探讨松果体及其褪黑素在大鼠胸腺T细胞分化发育中的作用。结果显示松果体摘除后4周时大鼠体重下降明显(P0.05),术后8周胸腺重量减轻显著。褪黑素补充能在一定程度上缓解松果体摘除引起的体重和胸腺重量下降趋势,高剂量组尤为明显。松果体摘除后8周胸腺皮质CD4~+阳性细胞面积减少具统计学意义,补充褪黑素后呈剂量依赖性恢复。髓质CD8~+细胞面积术后4周明显升高,补充褪黑素后略有下降,但其升高仍然具统计学意义。流式细胞术检测结果显示松果体摘除后4周胸腺内CD4~+细胞比例以及双阳性、双阴性细胞比例变化不明显,而CD8~+细胞比例则下降明显,至术后8周CD8~+细胞比例则上升,双阴性细胞比例降低。补充褪黑素4周时CD8~+细胞比例呈剂量依赖性升高,高剂量组无论4周还是8周均有显著性意义,双阳性细胞比例则降低。松
[Abstract]:Since Berman put forward the concept of pineal gland and thymus in 1926, many experimental studies have shown that there is an obvious functional relationship between pineal gland and thymus. Therefore, the relationship between pineal gland and thymus structure and function and its related mechanism are still unclear. In this study, the effects of pineal gland and melatonin on the differentiation and development of T cells in rat thymus were studied from the following four aspects using the rat model of pinealectomy. First, Effects of pineal gland and melatonin on the differentiation and development of T cells in rat thymus the different subtypes of pineal gland and melatonin on the differentiation and development of thymus T cells The influence of the group and its possible mechanism have not been reported. In this part, immunocytochemistry, computer image analysis and flow cytometry were used to detect T lymphocyte subsets in thymus and blood after pinealectomy and melatonin supplementation. To observe the changes of CD _ 4 / 8 subsets of T cells in thymus and blood after pinealectomy and the effects of melatonin supplementation on the differentiation and development of T cells in rat thymus. The results showed that the weight of the thymus decreased significantly at 4 weeks after pinealectomy (P0.05), and the weight of thymus decreased significantly at 8 weeks after operation. The decrease of weight and thymus weight caused by pinealectomy was alleviated to some extent by melatonin supplementation, especially in the high dose group. The area of CD4- positive cells in thymic cortex decreased significantly 8 weeks after pinealectomy and recovered in a dose-dependent manner after supplementation of melatonin. The area of CD8 ~ cells in medulla increased significantly 4 weeks after operation, and decreased slightly after melatonin supplementation, but the increase was still statistically significant. The results of flow cytometry showed that the percentage of CD4 ~ cells and double positive cells in thymus of pineal gland 4 weeks after pinealectomy did not change significantly, but the proportion of CD8 ~ cells decreased significantly, but the proportion of CD8 ~ cells increased at 8 weeks after operation. The proportion of double negative cells decreased. The percentage of CD8- cells increased in a dose-dependent manner at the 4th week after melatonin supplementation. In the high-dose group, there were significant differences in the ratio of CD8- cells at the 4th week and the 8th week, but the proportion of the double-positive cells decreased. Pine
【学位授予单位】：福建医科大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392

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