射频电磁场对人树突状细胞的表型和功能影响的体外研究

发布时间：2018-06-24 22:14

本文选题：树突细胞/免疫学 + 电磁场/副作用　；参考：《浙江大学学报(医学版)》2008年01期

【摘要】：目的:研究手机射频电磁辐照对人树突状细胞(DC)的表型和功能的影响,为评价手机射频电磁场对免疫系统功能的影响提供实验依据。方法:用比吸收率(SAR)为4 W/kg的1800 MHzGSM射频电磁场间断(5 min开10 min停模式)辐照DC细胞0 h、1 h、12 h或24 h,以流式细胞仪检测各处理组DC表面HLA-DR和协同刺激分子(CD80、CD86、CD40及CD11c)的变化;以CCK-8试剂检测各组DC的同种混合淋巴细胞反应(MLR)能力的差异;以ELISA双抗体夹心法检测各组DC分泌细胞因子IL-12p70、TNF-α功能的差异。结果:与假辐照组相比,各辐照处理组DC表型分子HLA-DR、CD80、CD86、CD40的阳性细胞百分率均明显下降(P0.05),但CD11c无明显变化;各辐照处理组DC的同种MLR能力明显降低(P0.05),尤其以24 h处理组为甚。各辐照处理组DC的IL-12p70、TNF-α的分泌无显著差异。结论:在本实验条件下,射频电磁场辐照对DC表面分子的表达及对同种T细胞的刺激能力具有一定的抑制作用。
[Abstract]:Aim: to study the effects of radiofrequency electromagnetic (RF) irradiation on the phenotype and function of human dendritic cells (DC) in order to provide experimental evidence for evaluating the effects of RF electromagnetic fields on immune system. Methods: DC cells were irradiated with 1800 MHz GSM RF electromagnetic field (5 min on 10 min stop mode) with a specific absorptivity of 4 W / kg for 12 h or 24 h. The changes of HLA-DR and CD40 and CD11c on DC surface were detected by flow cytometry. The ability of allogeneic mixed lymphocyte reaction (MLR) was detected by CCK-8 reagent and the function of IL-12p70 TNF- 伪 secreted by DC in each group was detected by Elisa double antibody sandwich method. Results: compared with the sham irradiation group, the percentage of HLA-DRCD80 CD86 + CD40 positive cells decreased significantly in each irradiation group (P0.05), but CD11c did not change significantly, and the MLR ability of DC in each irradiation group was significantly decreased (P0.05), especially in the 24 h treatment group. There was no significant difference in the secretion of IL-12p70 TNF- 伪 between the irradiated groups. Conclusion: the radiofrequency electromagnetic field irradiation can inhibit the expression of DC surface molecules and the stimulation ability of allogeneic T cells.
【作者单位】：浙江大学医学院免疫学研究所浙江大学医学院浙江省生物电磁学重点研究实验室浙江大学医学院免疫学研究所浙江大学医学院免疫学研究所浙江大学医学院免疫学研究所浙江大学医学院浙江省生物电磁学重点研究实验室浙江大学医学院免疫学研究所浙江大学医学院免疫学研究所
【基金】：国家自然科学基金资助项目(30471588)
【分类号】：R392

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