学习记忆能力与神经干细胞分裂增殖之间关系的初步研究

发布时间：2018-06-25 07:06

本文选题：学习 + 记忆　；参考：《广西医科大学》2006年硕士论文

【摘要】： 【目的】探讨不同的学习记忆能力与神经干细胞增殖之间的关系,并阐明星型胶质细胞在其中发挥的作用。【方法】以成年雄性昆明小鼠为研究对象,随机分为水迷宫训练组及非水迷宫训练组(对照组组);而水迷宫训练组动物则根据其在水迷宫训练中的表现,再分为三组:学习较强组、学习能力一般组和学习能力弱组。水迷宫训练的最后3d,所有实验动物均进行5-溴脱氧尿嘧啶腹腔注射。水迷宫训练后的第1、7、14天,分别从各组中抽取5只动物,灌注取脑切片后进行免疫组组织化学染色,以检测神经干细胞(NSCs)增殖及存活情况,及星形胶质细胞数目的变化。【结果】1、根据水迷宫训练的逃避潜伏期,将水迷宫训练组小鼠分为三组:学习能力强组(15只),学习能力一般组(15只),及学习能力弱组(18只)。2、免疫组织化学染色:⑴.训练后第1天,学习能力强组与学习能力一般组的BrdU阳性细胞数均明显多于对照组(P0.01,P0.05),并且,学习能力强组与学习能力一般组和学习能力弱组之间的差别也具有统计学意义(P0.01),而学习能力弱组与对照组之间的差别不明显(P0.05)。⑵.训练后第7天,水迷宫训练组动物齿状回的BrdU阳性细胞数明显多于对照组(P0.05或P0.01),且学习能较强组明显多于其它三组(P0.05或P0.01),而学习能力一般组与学习能力弱组之间无明显差异(P0.05)。⑶.训练后第14天,学习能力强组齿状回的BrdU阳性细胞数仍明显多于其它三组(P0.01);同时,学习能力一般组与其它两组之间的差异也具有统计学意义(P0.01);而学习能力弱组与对照组之间的差异不明显(P0.05)。⑷.训练后第1、7、14天,各组动物室管膜下区BrdU阳性细胞数没有明显差别(P0.05)。⑸.各组动物之间,齿状回及海马各区星型胶质细胞的数目及灰度没有明显差异(P0.05)。【结
[Abstract]:[objective] to explore the relationship between different learning and memory abilities and the proliferation of neural stem cells, and to elucidate the role of astrocytes in the process. [methods] the adult male Kunming mice were studied. They were randomly divided into water maze training group and non-water maze training group (control group), while water maze training group was divided into three groups according to their performance in water maze training: learning ability group, learning ability general group and learning ability weak group. In the last 3 days of water maze training, all experimental animals were intraperitoneally injected with 5-bromodeoxyuridine. On the 7th and 14th day after water maze training, 5 animals were extracted from each group, and then the brain sections were perfused for immunohistochemical staining to detect the proliferation and survival of neural stem cells (NSCs). And the number of astrocytes. [results] 1. According to the escape latency of water maze training, The mice in the water maze training group were divided into three groups: learning ability group (15 mice), learning ability general group (15 mice) and learning ability weak group (18 mice). On the first day after training, the number of BrdU positive cells in the learning ability group and the general learning ability group was significantly higher than that in the control group (P0.01P 0.05). The difference between the learning ability group and the general learning ability group and the learning ability weak group was also statistically significant (P0.01), but the difference between the learning ability weak group and the control group was not significant (P0.05). 2. 7 days after training, The number of BrdU positive cells in dentate gyrus in the water maze training group was significantly higher than that in the control group (P0.05 or P0.01), and the number of BrdU positive cells in the strong learning ability group was significantly higher than that in the other three groups (P0.05 or P0.01), but there was no significant difference between the general learning ability group and the weak learning ability group (P0.05) .3. On the 14th day after training, the number of BrdU positive cells in dentate gyrus in the learning ability group was significantly higher than that in the other three groups (P0.01). The difference between the general learning ability group and the other two groups was statistically significant (P0.01), but the difference between the learning ability weak group and the control group was not significant (P0.05). 4. There was no significant difference in the number of BrdU positive cells in the subependymal area of each group (P0.05). There was no significant difference in the number and grayscale of astrocytes among the groups, dentate gyrus and hippocampal regions (P0.05).
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R33

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