长期饮用硝酸钐对小鼠免疫功能影响的实验研究

发布时间：2018-06-25 17:10

本文选题：硝酸钐 + 免疫功能　；参考：《浙江师范大学》2006年硕士论文

【摘要】：目的：研究稀土的长期摄入对机体免疫功能是否存在影响，为制定稀土使用安全标准和评价稀土生物效应提供实验依据。本实验以不同剂量(5、50、500、2000mg／L)硝酸钐染毒3个月的ICR小鼠为对象，通过染毒过程中体重的动态变化、脏器指数、迟发型变态反应、E花结形成率、免疫球蛋白含量及腹腔巨噬细胞吞噬功能、红细胞受体花环研究了小鼠细胞免疫功能、体液免疫功能、巨噬细胞吞噬功能、红细胞免疫吸附功能的变化；另外通过对血清Sm浓度及部分与免疫功能密切相关的金属元素的浓度、血清抗氧化能力测定及红细胞形态观察初步探讨可能存在的硝酸钐免疫毒性机制。结果：1．一般体征检查结果表明小鼠在染毒过程中各组间体重无明显差异(P＞0.05)。雄鼠500mg·L~(-1)和2000mg·L~(-1)剂量组脾指数明显低于对照组(P＜0.05)，其它脏器指数与对照组相比无统计学意义，推测脾对硝酸钐作用更敏感。各剂量组免疫器官肉眼观察与对照组无明显区别；随染毒剂量增高，脾组织结构发生变化，超微结构观察发现淋巴细胞核形变、线粒体肿胀空化、核周间隙增宽、巨细胞增多甚至细胞解体。 2．雌鼠在50mg·L~(-1)、500mg·L~(-1)剂量组的足跖肿胀度明显低于对照组(P＜0.05)；而雄鼠除5mg·L~(-1)剂量组外，各组肿胀度均显著下降，，2000mg·L~(-1)剂量组肿胀度仅为对照组的60％。当硝酸钐浓度升高至2000mg·L~(-1)时，雌雄鼠的E花结形成率均被严重抑制(P＜0.01)，可见饮用硝酸钐损伤了机体建立特异性细胞免疫的能力。 3．雌鼠除5mg·L~(-1)组外，其余各组溶血空斑形成数显著下降(P＜0.01)，而雄鼠即使在最低剂量组PFC也出现明显变化(P＜0.05)。在一定浓度范围内(5～500mg·L~(-1))，硝酸钐可能会抑制更多抗体(IgG)产生，随着染毒剂量的增加，这种抑制作用减弱。硝酸钐的摄入对小鼠体液免疫应
[Abstract]:Objective: To study the effect of the long-term intake of rare earth on the immune function of the body, to provide an experimental basis for the formulation of the safety standard for the use of rare earth and the evaluation of the biological effect of rare earth. In this experiment, the ICR mice infected with samarium nitrate at different doses (5,505002000mg / L) were used for 3 months, through the dynamic changes of body weight and the organ index. The late onset allergy, the formation rate of E flower knot, the content of immunoglobulin and the phagocytosis of peritoneal macrophages. The erythrocyte receptor rosette studied the cellular immune function, the humoral immune function, the macrophage phagocytosis and the changes of the red cell immune adsorption function, and the serum Sm concentration and part of the immune function were closely related to the serum concentration and part of the serum. The concentrations of metal elements, serum antioxidant capacity and morphology of erythrocytes were preliminarily explored to explore the possible mechanism of immune toxicity of samarium nitrate.
Results: 1. the results of general physical examination showed that there was no significant difference in weight between the mice during the process of exposure (P > 0.05). The spleen index of the male rats 500mg L~ (-1) and 2000mg. L~ (-1) dose group was significantly lower than that of the control group (P < 0.05). The other visceral index had no statistical significance compared with the control group, and the spleen was more sensitive to samarium nitrate. There was no obvious difference between the immune organs and the control group. With the increase of the dose, the structure of the spleen was changed. The ultrastructural observation revealed the deformation of the lymphocyte nucleus, the swelling and cavitation of mitochondria, the widening of the perinuclear space, the increase of giant cells and even the disintegration of the cells.
The feet swelling degree of 2. female rats in 50mg. L~ (-1) and 500mg. L~ (-1) dose group was significantly lower than that of the control group (P < 0.05), but the swelling degree of the male rats was significantly lower than the 5mg. L~ (-1) dose group. The swelling degree of the 2000mg L~ dose group was only the control group when the concentration of samarium nitrate increased to the conclusion. Severely inhibited (P < 0.01), it is obvious that drinking samarium nitrate damages the body's ability to establish specific cellular immunity.
3. female rats in addition to 5mg L~ (-1) group, the formation of hemolytic plaque in the rest of the other groups decreased (P < 0.01), and the male rats had obvious changes (P < 0.05) even in the lowest dose group (P < 0.05). In a certain concentration range (5 to 500mg. L~ (-1)), samarium nitrate could inhibit more antibodies (IgG), and this inhibition weakened with the increase of the dose. The absorption of samarium nitrate to the humoral immune response in mice
【学位授予单位】：浙江师范大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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