大鼠直、结肠炎CLS的镇痛作用及COX2与GFAP在CNS表达的形态学观察

发布时间：2018-06-26 03:55

本文选题：化学性腰交感神经切除术(CLS) + COX_2　；参考：《新疆医科大学》2005年硕士论文

【摘要】：目的:研究化学性腰交感神经切除术(CLS)对直、结肠慢性刺激性炎症和扩张性伤害性刺激(CRD)的中枢保护作用,为临床医学提供基础研究资料。方法:Wistar大鼠54只,随机分为6组,每组9只,按二因素析因实验设置实验组,另外设立实验对照组和空白对照组。利用直、结肠慢性刺激性炎性致痛模型,切除模型大鼠的腰交感神经后,运用机械扩张刺激观察大鼠行为变化。用Miampamba评分方法改良后疼痛四级评分标准评分,重复3次取均值记录结果。取间脑、脑桥、延髓、颈段(C_(5-8))、胸段(T_(3-6))、腰段(L_(1-3))、骶段(S_(2-4))脊髓连续切片后,延髓和脊髓用环氧合酶2(COX2),间脑、脑桥用胶质纤维酸性蛋白(GFAP)免疫组化染色,显微镜下观察相应核团,计数阳性细胞数。结果:化学性腰交感神经切除术CLS无论作为预防性手术还是治疗性手术均能使中枢内GFAP和COX2表达阳性的细胞数下降(p0.05)。NST,C_(5-8)中间带和T_(3-6)中间带外侧核不是直、结肠慢性炎症刺激和CRD扩张性伤害刺激上传通路中的中继神经元(p0.05)。一、二、三、四级反射各组比较直、结肠扩张刺激阈值与手术顺序无关而与手术有关(p0.05)。假手术不影响痛觉阈值(p0.05),而手术后痛觉阈值明显上调(p0.05)。结论:化学性腰交感神经切除术(CLS)在直、结肠慢性炎症性刺激和CRD伤害刺激过程中能有效的保护中枢神经系统。腰交感神经可能是直、结肠伤害性刺激上传的传入神经。CLS的中枢保护作用与手术时间顺序无关。
[Abstract]:Objective: to study the central protective effect of chemical lumbar sympathectomy (CLS) on chronic irritant inflammation of colon and dilated nociceptive stimulation (CRD), and to provide basic data for clinical medicine. Methods Fifty-four Wistar rats were randomly divided into 6 groups with 9 rats in each group. The experimental group was set up according to the two-factor factorial experiment, and the experimental control group and the blank control group were set up. The model of pain induced by chronic irritation of colon and straight colon was used to observe the behavior of rats after the lumbar sympathetic nerve was excised and the mechanical dilatation was used to observe the behavior of the rats. The modified Miampamba scoring method was used to evaluate the four levels of pain. Diencephalon, pons, medulla oblongata, cervical segment (C5-8), thoracic segment (T3-6),) lumbar segment (L1-3), sacral segment (S2-4) spinal cord were sectioned continuously, the medulla oblongata and spinal cord were stained with cyclooxygenase-2 (COX2), diencephalon and pons were stained with glial fibrillary acidic protein (GFAP) immunohistochemical staining, and the corresponding nuclei were observed under microscope. Count the number of positive cells. Results: CLS could decrease the number of GFAP and COX2 positive cells in central nervous system (p0.05). NST-C5-8 and T3-6 medial zone were not straight. Relay neurons in the upload pathway of chronic inflammatory stimulation and CRD distensible nociceptive stimulation (p0.05). The first, second, third, and fourth reflex groups were straight, and the threshold of colon dilatation stimulation was not related to the operation order but to the operation (p0.05). The nociceptive threshold was not affected by sham operation (p 0.05), but increased significantly after operation (p 0.05). Conclusion: chemical lumbar sympathectomy (CLS) can effectively protect the central nervous system during chronic inflammatory stimulation and CRD nociceptive stimulation. Lumbar sympathetic nerve may be straight, the central protective effect of afferent nerve. CLS uploaded by colonic nociceptive stimulation is not related to the operation time sequence.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R361

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