中国部分地区艰难梭菌PCR-核糖体分型及毒素基因多态性研究

发布时间：2018-06-28 17:11

本文选题：艰难梭菌 + 艰难梭状芽孢杆菌　；参考：《中国感染控制杂志》2014年01期

【摘要】：目的了解中国部分地区艰难梭菌聚合酶链反应(PCR)-核糖体型别分布及其A、B毒素基因多态性,为建立适宜中国的艰难梭菌分子检测和分子分型技术提供基础数据,同时在基因水平上为艰难梭菌感染导致的复杂临床表现提供依据。方法对中国3个城市(北京、广州、济南)分离的64株艰难梭菌临床株进行PCR-核糖体分型,并对不同型别的26株代表菌株的A、B毒素基因进行扩增测序。结果 64株艰难梭菌中,毒素基因型以A+B+型(45株,70.31%)为主,A-B+型19株(29.69%)。共存在9种PCR-核糖体型别,以017型(21株,32.81%)为主要型别,其次为001型(13株,20.31%)、012型(11株,17.19%)。A-B+菌株中,14株(73.68%)是017型,1株是001型。A、B毒素基因呈现一定的多态性,其中有7种A毒素序列型别(TSTA),6种B毒素序列型别(TSTB),8种A、B毒素序列型别组合(TSTG)。结论我国部分地区的艰难梭菌可能以PCR-核糖体017型为主,A、B毒素基因在菌株间存在多态性,且核糖体型别与毒素基因多态性间存在相对应的关联。应进一步扩大菌株数量和范围,探寻适合我国的分子检测和分子分型方法,从而帮助医院更好地预防和控制艰难梭菌感染。
[Abstract]:Objective to investigate the distribution of polymerase chain reaction (PCR) ribosomal type and AmoB toxin gene polymorphism of Clostridium diffracta in some parts of China, and to provide basic data for establishing molecular detection and molecular typing techniques suitable for China. At the same time, at the gene level for the complex clinical manifestations caused by Clostridium difficulty infection to provide the basis. Methods PCR- ribosomal typing was performed on 64 clinical strains of Clostridium difficulty isolated from three cities in China (Beijing, Guangzhou, Jinan), and 26 representative strains of AGB toxin gene of different types were amplified and sequenced. Results among 64 strains of Clostridium difficulty, A B genotype (45 strains 70.31%) was the main genotype of toxin type A A B, 19 strains (29.69%) were serotype A B. There were 9 kinds of PCR- ribosome types, of which 017 (21 strains, 32.81%) were the main type, followed by 001 (13 strains, 20.31%), 012 (11 strains, 17.19%). Among them, there are 7 A toxin sequence types (TSTA) and 6 B toxin sequence types (TSTB) and 8 AZB toxin sequence combinations (TSTG). Conclusion the PCR- ribosomal 017 gene may be the main gene polymorphism of Clostridium difficulty in some areas of China, and there is a corresponding association between ribosomal type and toxin gene polymorphism. It is necessary to further expand the number and scope of strains and to explore molecular detection and molecular typing methods suitable for our country so as to help hospitals to prevent and control Clostridium difficult infection better.
【作者单位】：中国疾病预防控制中心;中南大学湘雅医院;
【基金】：国家自然科学基金(No.81101218)
【分类号】：R378.8

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