胎儿发育中弥散神经内分泌细胞的表达及其意义

发布时间：2018-06-30 00:48

本文选题：胎儿发育 + 弥散神经内分泌系统　；参考：《暨南大学》2005年硕士论文

【摘要】：目的: 检测胎儿发育中弥散神经内分泌细胞(diffuse neuroendocrine cell,简称DNES细胞)分布和表达特征,探讨DNES细胞对胎儿器官发生和分化过程的生物学作用及其意义。方法: 14～38周胎儿共12例,取用唾液腺、胰腺、胃、肺、肾和前列腺等器官组织,常规石蜡包埋和切片。HE染色作正常结构对照。免疫组织化学sP法第一抗体用DNES相关抗体:抗嗜铬素A(CgA)、抗神经元特异性烯醇化酶(NSE)、抗生长抑素(SS)、抗降钙素基因相关肽(CGRP)、抗巢蛋白(nestin)和抗胰岛素(insulin)等;链霉素抗生物素蛋白—过氧化酶(Streptavidin-Peroxidase,S-P)试剂盒反应后,DAB显色,苏木素复染。结果: 胎儿器官中CgA、NSE、nestin、SS和CGRP阳性细胞表达均十分活跃。DNES细胞表达特征:在胰腺DNES细胞由弥散分布逐渐集中到胰岛,外分泌部也有散在出现;在胎肺DNES细胞主要分布于呼吸道上皮;在唾液腺于导管上皮和间质中检出DNES细胞;在胃中DNES细胞主要集中胃腺;在肾脏DNES细胞表达较弱,在肾小管和肾小囊脏层可见;前列腺上皮中也已出现DNES细胞。随器官发育分化,上述5种抗体的阳性反应特征与胎儿弥散神经内分泌系统(diffuse neuroendocrine system,DNES)的形成和成熟分化及其功能意义相符合。结论: 与胎儿器官发育分化同步,DNES细胞发生发育活跃,虽来源于不同胚层器官且表达特征各有差异,但各类DNES细胞发生过程可能与神经性干细胞分化程序有渊源关系。胎儿器官中DNES细胞的弥散性分布特征和多样性作用方式,证实内分泌和外分泌细胞发生发育过程密切相关,并表明DNES细胞在胎儿发育过程的作用,可能涵盖神经系统调节与内分泌调节的意义。
[Abstract]:Objective: to detect the distribution and expression of diffuse neuroendocrine cells (diffuse neuroendocrine cell,) in fetal development. To investigate the biological effect and significance of DNES cells on fetal organogenesis and differentiation. Methods: a total of 12 fetuses, including salivary glands, pancreas, stomach, lung, kidney and prostate, were collected from 12 fetuses at the age of 14 weeks. Routine paraffin embedding and section. HE staining were used as normal structure control. The first antibody of immunohistochemistry SP method was DNES-related antibodies: anti-chromaffin A (CGA), anti-neuron-specific enolase (NSE), anti-somatostatin (SS), anti-calcitonin gene-related peptide (CGRP), anti-nestin (nestin) and anti-insulin (insulin). Streptavidin-peroxidase (S-P) reaction kit reacted with DAB and hematoxylin was restained. Results: the expression of SS and CGRP positive cells in fetal organs were very active. DNES cells were expressed in the pancreas. The distribution of DNES cells in the pancreas was gradually concentrated to the islets. DNES cells were mainly distributed in respiratory tract epithelium in fetal lung, DNES cells were detected in ductal epithelium and stroma in salivary gland, DNES cells were mainly concentrated in gastric gland, and DNES cells were weakly expressed in kidney. DNES cells were also found in renal tubules and renal vesicles. With the development and differentiation of organs, the positive reaction characteristics of the above five antibodies were consistent with the formation, maturation and differentiation of fetal diffuse neuroendocrine system (diffuse neuroendocrine) and its functional significance. Conclusion: DNES cells are active in the development and differentiation of fetal organs, although they are derived from different embryonic organs and have different expression characteristics. However, the development of DNES cells may be related to the neural stem cell differentiation process. The diffuse distribution and diversity of DNES cells in fetal organs confirmed that endocrine and exocrine cells were closely related to the development process and the role of DNES cells in fetal development. It may cover the significance of nervous system regulation and endocrine regulation.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R321

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