慢性阻塞性肺疾病寒饮蕴肺证大鼠模型的评价

发布时间：2018-07-03 02:07

本文选题：慢性阻塞性肺疾病 + 寒饮蕴肺证　；参考：《山东中医药大学》2007年硕士论文

【摘要】： 目的:从理论上,对慢性阻塞性肺疾病寒饮蕴肺证进行系统的阐释与论证,以丰富寒饮蕴肺证理论。利用熏烟、气管滴注脂多糖加寒凉刺激的方法建立慢性阻塞性肺疾病寒饮蕴肺证大鼠模型。对大鼠模型可信性作出评价;同时,观察温阳化饮方和布地奈德对大鼠慢性阻塞性肺疾病寒饮蕴肺证模型干预的效果。方法:依据“外内合邪”、“形寒饮冷则伤肺”等观点,结合慢性阻塞性肺疾病致病原因,在冬季寒冷环境中冷冻大鼠,大鼠饮水给以冰水,配以熏烟、气管滴注脂多糖的方法,建立慢性阻塞性肺疾病寒饮蕴肺证大鼠模型。观察其自愈情况,并用温阳化饮方和布地奈德干预。观察大鼠的一般情况、肺组织病理学改变,检测有关肺功能指标和血清白介素-8、白介素-10。结果:慢性阻塞性肺疾病寒饮蕴肺证大鼠模型的一般情况与寒饮蕴肺证的临床表现相符,各项指标的变化(除血清白介素-8和白介素-10外)也与慢性阻塞性肺疾病的指标变化相一致:大鼠体重减轻,吸气阻力和呼气阻力升高、肺顺应性下降,但血清白介素-8升高和血清白介素-10浓度与正常组相比无显著性变化(P0.05),病理切片显示肺组织出现炎性损伤。在撤除造模因素4周后,慢性阻塞性肺疾病寒饮蕴肺证模型大鼠的一般情况及各项指标没有明显好转。用温阳化饮方和布地奈德干预的模型大鼠症状好转,肺功能改善,血清白介素-8和血清白介素-10浓度与慢性阻塞性肺疾病寒饮蕴肺证模型大鼠的相比无显著性变化(P0.05),病理切片示炎性损伤明显减轻。结论:用本实验方法建立慢性阻塞性肺疾病寒饮蕴肺证大鼠模型是可行的。温阳化饮方和布地奈德在改善慢性阻塞性肺疾病寒饮蕴肺证模型大鼠的一般情况、肺功能和抑制气道炎症反应方面有比较肯定的疗效,二者疗效没有差别。
[Abstract]:Objective: in order to enrich the theory of cold drink accumulation lung syndrome in chronic obstructive pulmonary disease (COPD). The model of chronic obstructive pulmonary disease (COPD) was established by fumigation, endotracheal instillation of lipopolysaccharide and cold stimulation. To evaluate the credibility of rat model and observe the effect of Wenyanghua decoction and budesonide on the model of chronic obstructive pulmonary disease (COPD). Methods: according to the viewpoint of "external internal and external evil" and "cold form cold drink hurt lung", combined with the cause of chronic obstructive pulmonary disease (COPD), the rats were given ice water and fumigation in the cold environment in winter. The rat model of chronic obstructive pulmonary disease (COPD) was established by endotracheal instillation of lipopolysaccharide (LPS). Observe its self-healing, and use Wenyang decoction and budesonide intervention. Observe the general situation of rats, lung histopathological changes, detection of lung function indicators and serum interleukin-8, interleukin-10. Results: the general situation of the rat model of cold drink accumulation of lung syndrome in chronic obstructive pulmonary disease was consistent with the clinical manifestation of cold drink accumulation of lung syndrome. The changes (in addition to serum interleukin-8 and interleukin-10) were also consistent with the changes in chronic obstructive pulmonary disease: weight loss, increased inspiratory and expiratory resistance, and decreased lung compliance. However, the increase of serum interleukin-8 and the concentration of serum interleukin-10 had no significant change compared with the normal group (P0.05). Pathological sections showed inflammatory injury in lung tissue. After removing the model factors for 4 weeks, there was no obvious improvement in the general situation and various indexes of the model rats of chronic obstructive pulmonary disease model of cold drink accumulation of lung syndrome. The model rats treated with Wenyanghua decoction and budesonide improved their symptoms and lung function. Serum interleukin-8 and serum interleukin-10 levels were not significantly changed compared with chronic obstructive pulmonary disease model rats (P0.05), pathological sections showed that inflammatory injury was significantly alleviated. Conclusion: it is feasible to establish the model of chronic obstructive pulmonary disease (COPD) with cold drink syndrome. Wenyanghua decoction and budesonide have positive effects on improving the general situation, pulmonary function and inhibiting airway inflammation in rats with chronic obstructive pulmonary disease (COPD) model of cold drink accumulation of lung syndrome, but there is no difference between the two.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332;R259

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