FGF-1改构体对小鼠脾细胞增殖、凋亡、IL-2产生的影响

发布时间：2018-07-05 09:42

本文选题：改构酸性成纤维细胞生长因子 + 脾细胞　；参考：《暨南大学》2005年硕士论文

【摘要】：酸性成纤维细胞生长因子(acid fibroblast growth factor,aFGF或FGF-1)是成纤维细胞生长因子家族成员之一,是一种重要的生长因子。人FGF-1(hFGF-1)是一个17∽18kDa的非糖基化多肽,三胚层来源的细胞都可以表达。FGF-1的生物学效应非常广泛,在组织和器官发育、血管发生、血细胞生成、肿瘤发生、伤口愈合中等发挥重要的作用。FGF-1对免疫系统也有重要的影响,能提高多种刺激诱导的T细胞增殖、凋亡及细胞因子的产生。目的:本文主要探讨改构重组人酸性成纤维细胞生长因子(MrhFGF-1)对balb/c小鼠脾细胞增殖、凋亡和IL-2产生的影响。方法:1、采用~3H-TdR掺入的方法检测分析MrhFGF-1和野生型hFGF-1对脾细胞增殖的影响。实验组分为(1)对照组;(2)FGF-1(hFGF-1、rhFGF-1、MrhFGF-1)处理组,(3)ConA处理组;(4)ConA+FGF-1(hFGF-1、rhFGF-1、MrhFGF-1)处理组。2、用流式细胞仪定量分析MrhFGF-1对脾细胞的凋亡保护作用。实验组分为(1)对照组;(2)DEX处理组;(3)DEX+FGF-1(hFGF-1、rhFGF-1、MrhFGF-1)处理组。3、用HT-2细胞系通过细胞增殖方法间接检测小鼠脾细胞在受到不同刺激因子刺激后产生IL-2的水平。实验分组和细胞增殖实验分组相同。结果:结果表明,利用DNA重组技术构建的一种非促分裂人FGF-1突变体(MrhFGF-1)和野生型hFGF-1和rhFGF-1相比,对脾细胞的促细胞增殖能力明显降低,但其对细胞凋亡的保护作用和对IL-2产生的影响并无显著性变化,即FGF-1的细胞凋亡保护作用及对IL-2产生的影响并不随着促细胞增殖能力的降低而降低。结论:FGF-1的促细胞增殖能力和细胞凋亡保护及协同刺激脾细胞产生IL-2的的信号通路并非由同一信号通路来实现的。
[Abstract]:Acidic fibroblast growth factor (acid fibroblast growth factoraFGF or FGF-1) is a member of fibroblast growth factor family and an important growth factor. Human FGF-1 (hFGF-1) is a 17 ~ 18kDa non-glycosylated polypeptide. FGF-1 plays an important role in wound healing. FGF-1 also plays an important role in the immune system. FGF-1 can increase T cell proliferation, apoptosis and cytokine production induced by various stimuli. Aim: to investigate the effects of recombinant human acidic fibroblast growth factor (MrhFGF-1) on spleen cell proliferation, apoptosis and IL-2 production in balb/c mice. Methods the effects of MrhFGF-1 and wild-type hFGF-1 on the proliferation of splenocytes were detected by 3H-TdR incorporation. The experimental group was divided into (1) control group, (2) FGF-1 (hFGF-1rhFGF-1rhFGF-1) treated group, (3) Cona FGF-1 (hFGF-1rhFGF-1rhFGF-1) treated group, (4) Cona FGF-1 (hFGF-1rhFGF-1rhFGF-1) treatment group. The experimental group was divided into (1) control group, (2) DEX treatment group and (3) DEX FGF-1 (hFGF-1rhFGF-1rhFGF-1) treatment group. The experimental groups were the same as the cell proliferation groups. Results: compared with wild-type hFGF-1 and rhFGF-1, a non-mitogenic human FGF-1 mutant constructed by DNA recombination technique could significantly reduce the proliferation of spleen cells. However, the protective effect of FGF-1 on cell apoptosis and the effect on IL-2 production had no significant change, that is, the protective effect of FGF-1 on apoptosis and IL-2 production did not decrease with the decrease of the ability to promote cell proliferation. Conclusion the signal pathway of promoting cell proliferation and cell apoptosis protection and co-stimulating spleen cells to produce IL-2 is not realized by the same signal pathway.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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