两种HIV-1重组腺病毒疫苗（修饰型，野生型）的构建及免疫原性比较

发布时间：2018-07-07 19:28

本文选题：HIV-1 + 修饰型gagpo(lmodgagpol)　；参考：《吉林大学》2006年硕士论文

【摘要】：人类免疫缺陷病毒(HIV)是逆转录病毒科的一个毒种,它主要引起艾滋病,即获得性免疫缺陷综合征(acquired immunodeficiency syndrome,AIDS),这是一种以全身免疫系统严重损害为主要特征的传染性疾病。虽然近几年抗艾滋病药物的“鸡尾酒”疗法在发达国家有效地控制了HIV的蔓延,但是昂贵的价格,耐药病毒株的产生,长期用药的副作用以及最终无法彻底清除患者体内病毒等方面的不利因素表明,只有艾滋病疫苗才能真正有效地预防和控制艾滋病。所以艾滋病疫苗的研究势在必行。 HIV的结构基因gagpol(wtgagpol)是HIV基因组中比较保守的区域,也是我们选择抗原的主要对象。在我们的工作中,首先为了提高产物蛋白的表达量,我们消除了一些抑制HIV-gag-pol表达的因子,构建了密码子优化过的gagpol(modgagpol)基因,为了研究wtgagpol和modgagpol在实验动物体内诱导的免疫应答水平,我们选择了重组复制缺陷型腺病毒5型(rAdV-5)作为载体将wtgagpol及modgagpol基因导入动物体内,并探讨了两者体液免疫及细胞免疫的差别。以上两种疫苗的应用和免疫方案的研究,为我们进一步探索利用重组腺病毒载体来研究HIV-1疫苗打下了基础。
[Abstract]:Human immunodeficiency virus (HIV) is a virus of the family retroviridae. It mainly causes AIDS, or acquired immunodeficiency syndrome (acquired immunodeficiency syndrome), which is a infectious disease characterized by serious damage to the systemic immune system. Although the "cocktail" therapy of anti-AIDS drugs has effectively controlled the spread of HIV in developed countries in recent years, the high price, the production of drug-resistant strains of the virus, The side effects of long-term drug use and the unfavorable factors, such as the failure to eliminate the virus in patients' bodies, indicate that only AIDS vaccine can effectively prevent and control AIDS. Therefore, it is imperative to study HIV vaccine. HIV structural gene gagpol (wtgagpol) is a conservative region of the HIV genome, and is also the main target of our selection of antigens. In our work, first of all, in order to increase the expression of the product protein, we eliminated some factors that inhibit the expression of HIV-1 gag-pol, and constructed the codon optimized gagpol (modgagpol) gene. In order to study the level of immune response induced by wtgagpol and modgagpol in experimental animals, We selected recombinant replication-deficient adenovirus type 5 (rAdV-5) as a vector to transfer wtgagpol and modgagpol genes into animals, and discussed the difference between humoral immunity and cellular immunity. The application of the above two vaccines and the study of immunization schemes have laid a foundation for further exploring and using recombinant adenovirus vectors to study HIV-1 vaccine.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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