EDS-40玻璃化液冻贮小鼠及人2～8细胞胚胎的研究

发布时间：2018-07-09 09:01

本文选题：玻璃化技术 + 玻璃化溶液　；参考：《重庆医科大学》2005年硕士论文

【摘要】：目的设计研究 EDS-40 玻璃化液冻贮小鼠及人 2～8 细胞胚胎的效果。方法设计研究 EDS-40 玻璃化液。按随机分组原则,分为实验组和对照组,实验组(a)为 EDS-40 玻璃化液,对照组(b)为国际上公认的效果最好的 EFS-40 玻璃化液,对照组(c)为程序冷冻。 1.EDS-40 玻璃化液的玻璃化测试 2.鼠胚冻贮:将小鼠 2～8 细胞胚胎按上述原则随机分成 a、b、c三组,a 、b 两组在 25°C 室温下分别加入 EDS-40 和 EFS-40 玻璃化液,装管后迅速投入液氮中保存。C 组采用程序冷冻。冻贮 2～3 个月后复温,用培养液反复洗涤后移入培养孔板培养,观察胚胎存活及卵裂情况。实验结果用 SPSS11.5 软件进行统计学分析。 3.人胚冻贮:选用人 2～8 细胞胚胎,随机分组、冻贮、复温方法及统计学处理同鼠胚冻贮。结果 1.EDS-40 玻璃化液能达到玻璃化效果。 2.小鼠 2～8 细胞胚胎 a、b、c 三组的冻后存活率分别为 84.31%、77.98%及 69.23%;卵裂率分别为 79.41%、66.97%及 53.85 %。 3.人 2～8 细胞胚胎 a、b、c 三组的冻后存活率分别为 53.85%、68%及 57.14%;卵裂率分别为 26.9%、56%及 28.57%。结论 1.EDS-40 玻璃化液能够达到玻璃化效果,且在冷冻小鼠及人 2～8
[Abstract]:Objective to design and study the effects of EDS-40 vitrification solution on embryo storage in mouse and human 2o 8 cells. Methods EDS-40 vitrification solution was designed and studied. According to the principle of random grouping, the experimental group was divided into experimental group and control group. The experimental group (a) was EDS-40 vitrification solution, and the control group (b) was the best internationally recognized EFS-40 vitrification solution. (c) of control group was programmed freezing. 1. Vitrification test of EDS-40 vitrification solution 2. Mouse embryo cryopreservation: mouse 2o 8 cells embryos were randomly divided into three groups according to the above principle. EDS-40 and EFS-40 vitrification solution were added at 25 掳C room temperature respectively. The embryos were stored in liquid nitrogen. Group C was stored in liquid nitrogen by programmed freezing. The embryo survival and cleavage were observed after 3 months of frozen storage and repeated washing of the culture medium and then transferred into the culture hole plate. The results were analyzed by SPSS 11.5 software. Human embryo cryopreservation: the embryos of human 2o 8 cells were divided randomly, stored by freezing, rewarming and statistical treatment. Results 1. EDS-40 vitrification solution can achieve vitrification effect. 2. The survival rate was 77.98% and 69.23%, and the cleavage rate was 79.41% and 53.85%, respectively. The survival rate of the three groups were 53.85% and 57.14%, and the cleavage rates were 26.9% and 28.57%, respectively. Conclusion 1. EDS-40 vitrification solution can achieve vitrification effect, and it can be used in frozen mice and human 2G 8. 2.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：Q813;R321

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