狂犬病固定毒aG株在地鼠肾细胞内病毒抗原定位及病毒增殖形态学研究

发布时间：2018-07-09 22:53

本文选题：RVaG株 + PHKC　；参考：《吉林大学》2007年硕士论文

【摘要】： 狂犬病病毒属弹状病毒科的狂犬病毒属,它属于单链RNA病毒。是引起人畜共患性传染病-狂犬病的病原体。自70年代以来,有关狂犬病街毒株及固定毒株在动物体内及培养细胞内的病毒抗原的荧光抗体染色及其病毒超微结构的研究陆续有报道[1-3]。但广泛应用于生产狂犬细胞培养疫苗的固定毒aG株在原代地鼠肾细胞内病毒复制部位、病毒增殖动态、病毒的超微结构、病毒形态发生以及光镜下病毒抗原定位与电镜下病毒复制部位的关系等报道很少。掌握病毒感染细胞后的生物学过程对狂犬病的预防、诊断、治疗都有重要意义。另外这方面的基础理论资料对改进生产工艺,提高狂犬疫苗产量和质量也有指导意义,同时也为生产和检定部门提供敏感性高、特异性强,并且简单快速的狂犬病毒抗原检测方法。为此,本论文利用现代免疫酶组织化学及电镜技术进行了系统试验。本实验着重对免疫组织化学技术中常用的免疫荧光法和免疫酶组织化学法(ABC法)进行对比试验。并对光镜和电镜观察结果进行了比较。
[Abstract]:Rabies virus of the family Granuloviridae, belonging to a single-stranded RNA virus. Is the cause of zoonotic sexually transmitted diseases-rabies pathogens. Since the 1970s, the studies on fluorescent antibody staining and ultrastructure of rabies street virus strains and stationary strains in animal and cultured cells have been reported one after another. But it is widely used in producing rabies cell culture vaccine. The viral replication site, virus proliferation dynamics and virus ultrastructure of the virus in primary hamster kidney cells are the fixed virus AG strain, which is widely used in the production of rabies cell culture vaccine. There are few reports on the relationship between viral morphogenesis and the localization of virus antigen under light microscope and the replication site of virus under electron microscope. It is important to master the biological process of virus infection for the prevention, diagnosis and treatment of rabies. In addition, the basic theoretical data in this respect also have guiding significance for improving the production process and improving the production and quality of rabies vaccine, and also provide high sensitivity and specificity for the production and verification departments. And simple and rapid detection method of rabies virus antigen. Therefore, a systematic experiment was carried out by using modern immunoenzyme histochemistry and electron microscopy. In this experiment, the immunofluorescence method and the immuno-enzyme histochemical method (ABC method), which are commonly used in immunohistochemistry, were studied. The results of light microscope and electron microscope were compared.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R446.6;R392

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