基于蛋白芯片模式的TORCH感染检测系统的构建及优化研究

发布时间：2018-07-09 23:18

本文选题：TORCH + (弓形虫　；参考：《第四军医大学》2005年硕士论文

【摘要】：TORCH是一类引起新生儿出生缺陷的微生物的总称,分别代表弓形虫、风疹病毒、巨细胞病毒和单纯疱疹病毒Ⅱ型和其他一些感染因素。这五种微生物在孕期感染妇女可以引起流产、早产及胎儿生长迟缓和发育畸形。由TORCH感染引起的孕期感染是一种最常见的先天性感染性疾病。虽然TORCH感染在怀孕早期会有一些轻微症状,但是如何在孕早期就发现并给予合理治疗仍然是人们面临的一个巨大挑战。目前,在尚无有效疫苗和治疗方法出现以前,对于孕妇TORCH感染的早发现、早诊断、早预防成为主要途径。本课题将这TORCH感染所涉及的五种微生物蛋白质分子按预先设置的排列方式固定于载体表面形成微阵列,根据蛋白质分子间特异性结合的原理,通过免疫胶体金构建显色系统和冷CCD成像模式,实现生物分子的简单、准确、快速、大信息量的检测。从microbix公司购回五种微生物的灭活抗原物质,用考马斯亮蓝G250法测蛋白的含量;用ELISA法检测五种蛋白的效价;用吸收-抑制实验检测五种抗原的特异性。通过优
[Abstract]:TORCH is a class of microbes that cause birth defects. They represent Toxoplasma, rubella virus, cytomegalovirus and herpes simplex virus type II and other factors. These five microbes can cause abortion, premature birth, fetal growth retardation and developmental malformation in pregnant women. The pregnancy induced by TORCH infection Infection is one of the most common congenital infection diseases. Although TORCH infection has some mild symptoms in the early pregnancy, it is still a great challenge to find and give reasonable treatment in the early pregnancy. The early discovery of TORCH infection in pregnant women before the emergence of effective vaccines and treatments has been found. Early diagnosis and early prophylaxis are the main ways.
In this project, five kinds of microbiological proteins involved in the TORCH infection are fixed on the surface of the carrier to form microarrays. According to the principle of specific binding between proteins, the color system and cold CCD imaging mode are constructed by immuno colloid gold, and the simple, accurate, rapid and large information amount of the biomolecules is realized. Testing the inactivated antigen of five kinds of microbes from microbix company, the content of protein was measured by Coomassie brilliant blue G250 method, the titer of five proteins was detected by ELISA method, and the specificity of five antigens was detected by absorption inhibition test.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R346

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