人巨细胞病毒感染致动脉粥样硬化的分子机制的研究

发布时间：2018-07-10 01:58

本文选题：人巨细胞病毒 + 感染　；参考：《山东大学》2006年硕士论文

【摘要】：第一部分人巨细胞病毒感染对人脐静脉内皮细胞黏附分子和RAGE受体表达的影响目的观察人巨细胞病毒(Human cytomegalovirus，HCMV)感染对人脐静脉内皮细胞(Human umbilical vein endothelial cells，HUVEC)的细胞间黏附分子-1(Intercellular adhesion molecule-1，ICAM-1)、血管细胞黏附分子-1(Vascular cell adhesion molecule-1，VCAM-1)及糖基化终产物受体(the Receptor for advanced glycation end products，RAGE)时序性表达的影响，研究HCMV感染致动脉粥样硬化(Atherosclerosis，AS)的作用机制。方法用HCMV感染HUVEC，采用RT-PCR方法检测不同感染时段细胞ICAM-1、VCAM-1及RAGE的mRNA表达。结果 1．HCMV感染对HUVEC ICAM-1mRNA表达的影响 HCMV感染HUVEC细胞后，0h时ICAM-1mRNA有基础水平的表达，4h开始升高，8h表达量达高峰，12h开始回落，48h表达量仍维持在较高的水平，各时段与0h比较，均有统计学意义(P＜0.01)。 2．HCMV感染对HUVEC VCAM-1mRNA表达的影响 HCMV感染HUVEC细胞后，0h时VCAM-1mRNA有基础水平的表达，4h开始升高，8h时达高峰，12h开始回落，24h回落更明显，，与0h比较，均有统计学意义(P＜0.01)，48h接近0h表达水平(P＞0.05)。
[Abstract]:Effect of human cytomegalovirus infection on the expression of adhesion molecules and rage receptors in human umbilical vein endothelial cells Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (Vascular) induced by human cytomegalovirus (HCMV) infection in human umbilical vein endothelial cells were observed. The effects of cell adhesion molecule-1 (VCAM-1) and the receptor for advanced glycation end products rage on the temporal expression of VCAM-1, To study the mechanism of atherosclerosis induced by HCMV infection. Methods HUVECs were infected with HCMV, and the mRNA expression of ICAM-1VCAM-1 and rage were detected by RT-PCR. The effect of HCMV infection on the expression of ICAM-1 mRNA in HUVEC The expression of ICAM-1 mRNA at 0 h after HUVEC increased at 4 h and reached the peak at 12 h and decreased at 48 h. The expression of ICAM-1 mRNA remained at a higher level at 48 h. Compared with 0h, 2. The effect of HCMV infection on the expression of VCAM-1 mRNA in HUVEC After HCMV was infected with HUVEC cells, the expression of VCAM-1 mRNA at 0 h after HCMV infection began to increase at 4 h and reached the peak at 12 h, and decreased at 12 h and decreased significantly at 24 h after HCMV infection, and the expression of VCAM-1 mRNA increased at 4 h after HCMV infection. Compared with 0 h, there was significant difference (P < 0.01) between 0 h and 48 h (P > 0.05).
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R373

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