华中地区I型人类免疫缺陷病毒（HIV-1）分子流行病学及耐药基因型的研究

发布时间：2018-07-21 19:36

【摘要】：1.我国中部河南省和湖北省有偿供血人群中HIV-1的分子流行病学研究自从HIV-1传入我国以来在我国的传播速度速度日益增长,范围越来越广。在河南和湖北等省许多地区,通过有偿供血感染HIV-1的人群引起了社会各界的广泛关注。为了研究河南和湖北省有偿供血人群中流行的HIV-1的遗传背景,我们对在2000年-2004年收集的标本进行了分子流行病学的分析。在收集的标本中69份来自河南和湖北省不同地区的有偿供血人员。通过对HIV-1gag的系统进化分析,我们发现在这两省有偿供血人群中流行的HIV-1遗传距离非常接近,河南省内流行的为3.8±0.8,湖北的为4.2±0.9,小于云南省的5.6,并且都与以前在云南省发现的YN.RIA2毒株相近,都为B'亚型病毒。对病毒的env以及pol片段的分析也显示了同样的结果。而且在进化树上我们也没有看到病毒根据不同地区聚集在进化树不同分枝的现象。我们的结果显示,B~'亚型病毒是河南和湖北省有偿供血人群中流行的主要病毒株,其来源相同,很可能是由云南传入,而且传入河南和湖北省的时间可能要早于07_BC和08_BC重组型病毒在云南德宏地区形成的时间。与此同时我们也对北京、新疆等地流行的HIV-1做了分析。与在有偿供血人群中流行的HIV-1不同,北京通过性传播感染的HIV-1主要是欧美B亚型病毒,新疆吸毒人群中流行的主要是07_BC重组型病诲。
[Abstract]:1. Molecular Epidemiology of HIV-1 among paid Blood donors in Henan Province and Hubei Province in Central China the spread of HIV-1 in China has been increasing since it was introduced into China. The scope is getting wider and wider. In many areas of Henan and Hubei provinces, people infected with HIV-1 through paid blood supply have attracted wide attention from all walks of life. In order to study the genetic background of HIV-1 in the paid blood donors of Henan and Hubei Province, we analyzed the molecular epidemiology of the samples collected from 2000 to 2004. Of the collected specimens, 69 were paid blood donors from different areas of Henan and Hubei provinces. Based on the phylogenetic analysis of HIV-1 gag, we find that the genetic distance of HIV-1 is very close among the paid blood donors in these two provinces. The prevalence of YN.RIA2 in Henan Province was 3.8 卤0.8, while that in Hubei was 4.2 卤0.9, which was smaller than that in Yunnan Province (5.6%), and were similar to the YN.RIA2 strains previously found in Yunnan Province, and were all B'subtype viruses. Analysis of env and pol fragments of the virus showed the same result. Moreover, we do not see the virus clustered in different branches of the evolutionary tree according to different regions in the evolutionary tree. Our results show that the virus is the main virus strain in the paid blood supply population in Henan and Hubei Province, and its origin is the same, which is probably introduced from Yunnan. Moreover, the time of transmission to Henan and Hubei Province may be earlier than that of 07C and 08SZ recombined virus in Dehong region of Yunnan Province. At the same time, we also analyzed the prevalence of HIV-1 in Beijing, Xinjiang and other places. Different from HIV-1 which is prevalent in paid blood supply population, HIV-1 infected by sexual transmission in Beijing is mainly subtype B virus in Europe and America, and in drug users in Xinjiang, it is mainly the 07BC recombinant disease.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R373

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