玻璃微流控芯片表面处理及其在DNA分析中的应用
发布时间：2018-07-24 16:44
【摘要】：玻璃微流控芯片表面处理及其在DNA分析中的应用 前言 微型全分析系统(Miniaturized Total Analysis Systems,μTAS)是当前世界上最前沿的科技领域之一,其目的是通过化学分析设备的微型化和集成化,最大限度地把分析实验室的功能集成到便携的分析设备中,甚至是方寸大小的芯片上,即“Lab on a chip”。微流控芯片是μTAS当前最活跃的研究领域,以微通道网络及众多分析功能元件的集成化为其结构特征,不仅使试样和试剂的消耗显著下降,而且具有高效、高速、高通量的分离分析能力。 生物医学是当前微流控芯片的主要应用领域。芯片毛细管电泳是以微流控芯片上的微通道作为分离通道的分离分析技术。因微通道比表面积显著增大,焦耳热能很快向四周溢散,所以可施加平板凝胶电泳难以达到的高场强,从而实现对样品的快速、高效的分离检测。 微流控芯片的微米级结构显著增大了微通道的比表面积,所以保持其内表面的物理化学性质的平衡、稳定具有十分重要的意义。 玻璃具备优良的光学性能,且易于进行表面改性,是最广泛使用的芯片基材之一。玻璃微流控芯片在进行电泳分析时,因微通道内表面—SiOH基团上的H~+解离,当在微通道两端施加外电场时,通道内整个缓冲溶液会流向阴极,形成电渗流(Electroosmotic Flow,EOF)。EOF不仅导致微通道表面对DNA样品的吸附而且严重影响玻璃芯片对DNA样品的分离效能,所以有效抑制EOF是玻璃芯片获得高分离效率的前提。 对芯片微通道进行表面改性处理是一种控制EOF的常用方法,可分为动态涂层和永久表面改性。前者是最简单的表面改性方法,改性化合物可通过物理吸附结合在微通道表面实现动态涂层。但因表面涂层不能长期保持稳定状态,其应用受到限制。后者是控制EOF和减少样品—表面吸附的最有效的方法。硅烷化试剂常用于永久改性处理中,其与微通道表面—SiOH基团可共价键合,也可进一步将线性聚合物交联固定在通道表面形成
[Abstract]:Surface treatment of Glass Microfluidic Chip and its Application in DNA Analysis Analysis Systems, 渭 TAS is one of the most advanced fields of technology in the world. Its aim is to integrate the function of the analysis laboratory into the portable analysis equipment, even on the chip of square inch, that is, "Lab on a chip", through the miniaturization and integration of the chemical analysis equipment. Microfluidic chip is the most active research field in 渭 TAS at present. With the integration of microchannel network and many analytical functional components as its structural characteristics, the consumption of samples and reagents is significantly reduced, and the microfluidic chip has high efficiency and high speed. High throughput separation and analysis capability. Biomedicine is the main application field of microfluidic chip. Chip capillary electrophoresis (CE) is a separation analysis technique using microchannels on microfluidic chips as separation channels. Because the specific surface area of the microchannel is significantly increased and the Joule heat energy spills out rapidly, the high field strength which is difficult to achieve by plate gel electrophoresis can be applied to realize the rapid and efficient separation and detection of the samples. The micron structure of the microfluidic chip increases the specific surface area of the microchannel significantly, so it is of great significance to maintain the balance and stability of the physical and chemical properties of the inner surface of the microfluidic chip. Glass with excellent optical properties and easy surface modification is one of the most widely used chip substrates. When the glass microfluidic chip is analyzed by electrophoretic analysis, the whole buffer solution in the microchannel will flow to the cathode when the external electric field is applied to the two ends of the microchannel because of the dissociation of H ~ on the inner surface of the microchannel. The formation of electroosmotic flow (Electroosmotic flow EOF). EOF not only results in the adsorption of DNA on the surface of microchannels, but also seriously affects the separation efficiency of glass chips to DNA samples. Therefore, it is a prerequisite for glass chips to obtain high separation efficiency by effectively inhibiting EOF. Surface modification of microchannel is a common method to control EOF, which can be divided into dynamic coating and permanent surface modification. The former is the simplest surface modification method, and the modified compounds can be dynamically coated on the microchannel surface by physical adsorption. However, its application is limited because the surface coating can not be kept stable for a long time. The latter is the most effective method for controlling EOF and reducing sample-surface adsorption. Silanization reagents are often used in permanent modification treatment. They can be covalently bonded with the -SiOH group on the surface of the microchannel, and further, the linear polymer crosslinking can be fixed on the surface of the channel to form.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R346
[Abstract]:Surface treatment of Glass Microfluidic Chip and its Application in DNA Analysis Analysis Systems, 渭 TAS is one of the most advanced fields of technology in the world. Its aim is to integrate the function of the analysis laboratory into the portable analysis equipment, even on the chip of square inch, that is, "Lab on a chip", through the miniaturization and integration of the chemical analysis equipment. Microfluidic chip is the most active research field in 渭 TAS at present. With the integration of microchannel network and many analytical functional components as its structural characteristics, the consumption of samples and reagents is significantly reduced, and the microfluidic chip has high efficiency and high speed. High throughput separation and analysis capability. Biomedicine is the main application field of microfluidic chip. Chip capillary electrophoresis (CE) is a separation analysis technique using microchannels on microfluidic chips as separation channels. Because the specific surface area of the microchannel is significantly increased and the Joule heat energy spills out rapidly, the high field strength which is difficult to achieve by plate gel electrophoresis can be applied to realize the rapid and efficient separation and detection of the samples. The micron structure of the microfluidic chip increases the specific surface area of the microchannel significantly, so it is of great significance to maintain the balance and stability of the physical and chemical properties of the inner surface of the microfluidic chip. Glass with excellent optical properties and easy surface modification is one of the most widely used chip substrates. When the glass microfluidic chip is analyzed by electrophoretic analysis, the whole buffer solution in the microchannel will flow to the cathode when the external electric field is applied to the two ends of the microchannel because of the dissociation of H ~ on the inner surface of the microchannel. The formation of electroosmotic flow (Electroosmotic flow EOF). EOF not only results in the adsorption of DNA on the surface of microchannels, but also seriously affects the separation efficiency of glass chips to DNA samples. Therefore, it is a prerequisite for glass chips to obtain high separation efficiency by effectively inhibiting EOF. Surface modification of microchannel is a common method to control EOF, which can be divided into dynamic coating and permanent surface modification. The former is the simplest surface modification method, and the modified compounds can be dynamically coated on the microchannel surface by physical adsorption. However, its application is limited because the surface coating can not be kept stable for a long time. The latter is the most effective method for controlling EOF and reducing sample-surface adsorption. Silanization reagents are often used in permanent modification treatment. They can be covalently bonded with the -SiOH group on the surface of the microchannel, and further, the linear polymer crosslinking can be fixed on the surface of the channel to form.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R346
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