TRAF2 K48聚泛素化位点的研究
[Abstract]:Objective to screen the main modification sites of K48 polyubiquitin chain on tumor necrosis factor receptor related factor 2 (tumor necrosis factor receptor-associated facto r 2 T RA F 2. Methods the amino acid sequences of T RA F 2 in different species were compared, and lysine with a conservative rate of more than 90% on human T RA F 2 was selected. The expression vector of human wild-type TRAF2 and the mutant expression vector of conserved lysine to arginine were constructed. Different TRAF2 expression vectors and NF- 魏 B luciferase reporter gene expression vectors were co-transfected into 293FT cells, and the activation of NF- 魏 B was detected by luciferase. Results there were 8 lysines with a conservative rate of more than 90% in human TRAF2. Restriction endonuclease digestion and sequencing showed that TRAF2 wild-type and mutant expression vector. NF- 魏 B luciferase reporter gene was successfully constructed in this study. The results showed that TRAF2-K320 may be the main modification site of K48 polyubiquitin chain. Conclusion TRAF2-K320 site plays a negative role in the activation of NF- 魏 B mediated by TRAF2.
【作者单位】: 同济大学医学院免疫学教研室;
【基金】:国家自然科学基金(31170824)
【分类号】:R3416
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