SP及其NK-1受体促进大鼠伤害性传入引起的脊髓后角星形胶质细胞的激活

发布时间：2018-07-31 11:49

【摘要】：疼痛是临床常见症状,给病人带来极大痛苦。新近研究表明脊髓胶质细胞在病理性痛的产生和维持中发挥重要作用。例如,皮下注入福尔马林、酵母聚糖、蛇毒等多种疼痛模型中,均可发现脊髓后角星形胶质细胞激活,使其特异性标记物胶质纤维酸性蛋白(glia fibrillary acidic protein, GFAP)表达增多。鞘内注射胶质细胞抑制剂(CNI-1493、Propentofy-lline)可阻断皮下注射福尔马林、酵母细胞壁等多种伤害性刺激引起的热痛敏和机械性痛敏。在病理性痛刺激状况下,激活的胶质细胞可释放一系列的神经活性物质(如白三烯、前列腺素、兴奋性氨基酸等)和促炎性细胞因子(如白细胞介素-1、白细胞介素-6、肿瘤坏死因子等)。这些活性物质一方面作用于脊髓后角的痛觉传递神经元使其处于敏化状态,另一方面促进初级传入纤维终末伤害性神经递质的释放。这些结果表明脊髓胶质细胞的激活在病理性痛及痛觉过敏的产生和维持中具有重要的作用。但是,病理性及痛过敏过程中脊髓胶质细胞激活的机制尚有待于阐明。 SP 是脊髓伤害性信息传递中重要的神经递质,它通过神经激肽-1(neurokinin -1, NK-1)受体参与脊髓伤害性信息的传递。研究发现,星形胶质细胞上有NK-1 受体的表达,SP 可促进星形胶质细胞瘤细胞DNA 的合成,并可诱导星形胶质
[Abstract]:Pain is a common clinical symptom, which brings great pain to patients. Recent studies have shown that glial cells play an important role in the generation and maintenance of pathological pain. For example, subcutaneous injection of formalin, yeast polysaccharide, snake venom and other pain models can be found in the spinal dorsal horn astrocytes activated, making its specific marker glial fibrillary acidic protein (GFAP) (glia fibrillary acidic protein, GFAP) expression increased. Intrathecal injection of glial cell inhibitor (CNI-1493 Propentofy-lline) can block the thermal and mechanical pain sensitivity caused by subcutaneous injection of formalin and yeast cell wall and other noxious stimuli. Activated glial cells release a range of neuroactive substances (such as leukotriene, prostaglandin, etc.) during pathological pain stimulation. Excitatory amino acids and proinflammatory cytokines (e. G. Interleukin-1, interleukin-6, tumor necrosis factor, etc.) On the one hand these active substances act on the nociceptive transmission neurons in the posterior horn of the spinal cord and on the other hand promote the release of nociceptive neurotransmitters at the primary afferent fiber terminals. These results suggest that the activation of glial cells plays an important role in the generation and maintenance of pathological pain and hyperalgesia. However, the mechanism of the activation of spinal glial cells in the process of pathophysiology and hyperalgesia remains to be elucidated. Sp is an important neurotransmitter in the transmission of nociceptive information in the spinal cord. It participates in the transmission of nociceptive information in spinal cord through neurokinin-1 (NK-1) receptor. It was found that the expression of NK-1 receptor on astrocytes and SP could promote the synthesis of DNA in astrocytoma cells and induce astrocytes.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R363

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