胆管结扎大鼠肠道菌群结构的分子生态学研究
发布时间：2018-10-17 07:59
【摘要】： 阻塞性黄疸(简称阻黄)是由于肝内、外胆管的机械性因素造成的胆汁瘀积、继而导致全身多系统病变的临床病症。阻黄及其引起的并发症与肠道菌群密切相关。本文采用常规的胆管结扎方法对SD大鼠进行阻黄造模,主要对手术前后的肠道菌群结构的变化进行了分子生态学研究,并与假手术组大鼠进行比较。采集胆管结扎组(n=5)及假手术组(n=5)大鼠手术前3天,手术后3天、7天、两周共四个时间点的粪便样品,提取粪便样品的总DNA,采用ERIC-PCR指纹图谱、16S rRNA基因V3区PCR-DGGE和针对4类肠道主要菌群(乳酸菌、梭菌、双歧杆菌、拟杆菌)的类群特异性PCR- DGGE并结合主成分分析方法对样品微生物结构组成进行深入分析。 ERIC-PCR分析结果表明,大鼠肠道菌群的组成具有宿主专一性,模型组大鼠肠道微生物群落多样性于胆管结扎后7天明显下降,原来的优势条带更加明显,而假手术组未出现此类情况。 对胆管结扎组与假手术组大鼠肠道菌群的16S rRNA基因V3区DGGE及其主成分分析(PCA)表明:(1)手术前大鼠肠道主要菌群的组成虽然具有个体差异性,但基本上聚在一起,并明显区分于手术后的大鼠。(2)手术后两周,胆管结扎组大鼠肠道主要菌群发生了不同于假手术组的变
[Abstract]:Obstructive jaundice is a clinical disease caused by mechanical factors of intrahepatic and extrahepatic bile duct, which leads to systemic multisystem lesions. The obstruction of yellow and its complications are closely related to the intestinal flora. In this paper, a routine bile duct ligation method was used to model SD rats. The changes of intestinal flora structure before and after operation were studied in molecular ecology, and compared with those in sham operation group. The fecal samples were collected from rats in bile duct ligation group (nong5) and sham operation group (nong5) 3 days before operation, 3 days after operation, 7 days after operation and 2 weeks. Total DNA, of fecal samples was extracted by ERIC-PCR fingerprinting, 16s rRNA V3 PCR-DGGE and four main intestinal flora (lactic acid bacteria, Clostridium spp., Bifidobacterium). Group specific PCR- DGGE and principal component analysis (PCA) were used to analyze the microbial structure of the samples. The results of ERIC-PCR analysis showed that, The composition of intestinal flora of rats was host specific. The diversity of intestinal microbial communities in the model group decreased significantly 7 days after bile duct ligation, and the original dominant bands were more obvious than those in the sham operation group. The DGGE of the V3 region of 16s rRNA gene in the intestinal flora of rats in bile duct ligation group and sham operation group and its principal component analysis (PCA) showed that: (1) although the composition of main intestinal flora of rats before operation had individual differences, it was basically clustered together. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R378;R575
本文编号：2276022
[Abstract]:Obstructive jaundice is a clinical disease caused by mechanical factors of intrahepatic and extrahepatic bile duct, which leads to systemic multisystem lesions. The obstruction of yellow and its complications are closely related to the intestinal flora. In this paper, a routine bile duct ligation method was used to model SD rats. The changes of intestinal flora structure before and after operation were studied in molecular ecology, and compared with those in sham operation group. The fecal samples were collected from rats in bile duct ligation group (nong5) and sham operation group (nong5) 3 days before operation, 3 days after operation, 7 days after operation and 2 weeks. Total DNA, of fecal samples was extracted by ERIC-PCR fingerprinting, 16s rRNA V3 PCR-DGGE and four main intestinal flora (lactic acid bacteria, Clostridium spp., Bifidobacterium). Group specific PCR- DGGE and principal component analysis (PCA) were used to analyze the microbial structure of the samples. The results of ERIC-PCR analysis showed that, The composition of intestinal flora of rats was host specific. The diversity of intestinal microbial communities in the model group decreased significantly 7 days after bile duct ligation, and the original dominant bands were more obvious than those in the sham operation group. The DGGE of the V3 region of 16s rRNA gene in the intestinal flora of rats in bile duct ligation group and sham operation group and its principal component analysis (PCA) showed that: (1) although the composition of main intestinal flora of rats before operation had individual differences, it was basically clustered together. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group. (2) two weeks after operation, the main intestinal flora in bile duct ligation group was different from that in sham operation group.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R378;R575
【引证文献】
相关期刊论文 前1条
1 李文宾;吴敏丽;廉振民;;分子生态学应用研究现状[J];中国微生态学杂志;2009年01期
，本文编号：2276022
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