人端粒酶逆转录酶基因转染U2OS细胞对其基质金属蛋白酶-2活性的影响

发布时间：2018-10-19 12:56

【摘要】： 目的:观察hTERT基因转染U2OS细胞对其基质金属蛋白酶2和9的影响,探讨hTERT基因和端粒酶的相关性。方法:RT-PCR和TRAP-ELISA法检测和明确对U2OS细胞hTERT基因的转染效果和转染阳性细胞克隆的筛选;明胶酶谱法和RT-PCR检测转染hTERT基因对细胞MMP-2,MMP-9表达和活性的影响。结果:hTERT基因转染U2OS细胞后,细胞成功的出现hTERT mRNA和明显的端粒酶活性(p0.05),使该细胞分泌酶原型MMP-(272KD)降低(p0.05),MMP-9完全消失。结论:hTERT稳定转染U2OS细胞克隆株,使细胞分泌酶原型MMP-9完全消失、酶原型MMP-2明显减少。但MMP-2在mRNA未见明显改变。
[Abstract]:Aim: to investigate the effect of hTERT gene transfection on matrix metalloproteinase 2 and 9 in U2OS cells and to explore the correlation between hTERT gene and telomerase. Methods: RT-PCR and TRAP-ELISA methods were used to detect the transfection effect of hTERT gene and the screening of positive cell clones in U2OS cells, and the effects of hTERT gene transfection on MMP-2,MMP-9 expression and activity were detected by gelatin zymography and RT-PCR. Results: after transfection of hTERT gene into U2OS cells, hTERT mRNA and telomerase activity (p0.05) were found successfully, MMP- (272KD) was decreased (p0.05) and MMP-9 disappeared completely. Conclusion: stable transfection of hTERT into U2OS cell clone completely disappeared the original MMP-9 of the cell secretory enzyme and significantly decreased the original MMP-2 of the enzyme. But MMP-2 did not change obviously in mRNA.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R363

【引证文献】