日本血吸虫感染对过敏性哮喘影响的实验研究

发布时间：2018-11-06 19:45

【摘要】：目的建立日本血吸虫感染并发过敏性哮喘实验动物模型及探讨日本血吸虫感染对过敏性哮喘的作用。方法取25只BALB/c小鼠,随机分为3组,A组为日本血吸虫感染并发过敏性哮喘组,B组为单纯过敏性哮喘组,C组为健康对照组。A组小鼠经腹部皮肤感染日本血吸虫尾蚴(23±3)条。8周后,以卵白蛋白(OVA)分别对A组、B组小鼠进行致敏激发,建立过敏性哮喘模型。12周后,取小鼠脾脏制成单细胞悬液并培养72 h,收集脾细胞上清液,检测细胞因子IL-4和IFN-γ水平;收集支气管肺泡灌洗液(BALF),观察嗜酸性粒细胞(EOS)所占的百分比;取小鼠肺组织作病理切片,观察小鼠肺组织的炎症变化。结果与B组比较,A组小鼠BALF涂片中EOS数减少(P0.05),A、B两组EOS百分比分别为(7.30±5.01)%和(43.60±2.53)%,而C组小鼠BALF中仅见少数气管上皮细胞,无炎细胞;ELISA法检测A、B、C组小鼠脾细胞培养上清IL-4水平分别为(96.02±38.54)pg/ml(、414.86±175.12)pg/ml和(13.55±1.66)pg/ml,IFN-γ水平分别为(11.60±4.18)pg/ml、(7.43±3.60)pg/ml和(7.55±1.57)pg/ml。与B组比较,A组小鼠IL-4水平降低(P0.05),IFN-γ水平无显著性变化(P0.05),IL-4/IFN-γ比值降低(P0.05)。肺部病理改变A组小鼠较轻,B组较重,C组无炎症反应。结论日本血吸虫感染对过敏性哮喘的发生有抑制作用。
[Abstract]:Objective to establish an experimental animal model of Schistosoma japonicum infection complicated with allergic asthma and to investigate the effect of Schistosoma japonicum infection on allergic asthma. Methods Twenty-five BALB/c mice were randomly divided into three groups: group A (Schistosoma japonicum infection complicated with allergic asthma) and group B (simple allergic asthma). The mice in group C were infected with cercariae of Schistosoma japonicum through abdominal skin (23 卤3). After 8 weeks, the mice in group A and group B were sensitized with ovalbumin (OVA), and the allergic asthma model was established 12 weeks later. The spleen of mice was isolated and cultured for 72 hours. The supernatants of spleen cells were collected and the levels of cytokines IL-4 and IFN- 纬 were detected. Bronchoalveolar lavage fluid (BALF),) was collected to observe the percentage of eosinophil (EOS), and the lung tissue was taken as pathological section to observe the inflammatory changes of mouse lung tissue. Results compared with group B, the number of EOS in BALF smear of group A was decreased (P0.05), the percentage of EOS in group A was (7.30 卤5.01)% and (43.60 卤2.53)%, while in group C, only a few tracheal epithelial cells were found in BALF. No inflammatory cells; The levels of IL-4 in the supernatant of splenocytes cultured in group A were (96.02 卤38.54) pg/ml (, 414.86 卤175.12) pg/ml and (13.55 卤1.66) pg/ml, respectively. IFN- 纬 levels were (11.60 卤4.18) pg/ml, (7.43 卤3.60) pg/ml and (7.55 卤1.57) pg/ml., respectively. Compared with group B, the level of IL-4 in group A decreased (P0.05), the level of IFN- 纬 did not change significantly (P0.05), and the ratio of IL-4/IFN- 纬 decreased (P0.05). Lung pathological changes were mild in group A, severe in group B, and no inflammatory reaction in group C. Conclusion Schistosoma japonicum infection can inhibit the occurrence of allergic asthma.
【作者单位】：天津医科大学寄生虫学教研室天津医科大学寄生虫学教研室天津医科大学寄生虫学教研室天津医科大学寄生虫学教研室天津医科大学寄生虫学教研室
【基金】：国家自然科学基金项目(No.30671830)
【分类号】：R725.6;R725.3

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