冠心病心绞痛寒凝血瘀证动物模型的建立

发布时间：2018-11-29 13:55

【摘要】：目的:探讨冠心病心绞痛寒凝血瘀证动物模型的建立方法并进行证候属性评价。方法:采用垂体后叶素单一因素大剂量静脉注射的方法建立冠心病心绞痛动物模型,通过观察大鼠一般情况、心电图和心肌微循环等指标变化,对模型的证候属性进行评价。结果:与正常组比较,模型组大鼠出现唇周、爪子颜色紫暗、体温下降等体征;心电图显示ST段显著抬高、心率减慢;心肌活体微循环检测显示,心肌微静脉收缩,红细胞流速明显减慢;血浆TXB2/6-Keto-PGF1α值显著升高(P0.05或P0.01)。结论:通过对模型宏观表征和微观指标的辨证分析,认为冠心病心绞痛寒凝血瘀证动物模型成功建立。这种在西医疾病基础上进行证候属性判定方法构建的病证结合模型客观性强、可信度高,更符合临床诊疗思路。
[Abstract]:Objective: to explore the establishment of animal model of angina pectoris with cold coagulation and blood stasis syndrome and to evaluate the syndromes. Methods: the animal model of coronary heart disease angina pectoris was established by single factor large dose intravenous injection of pituitrin. The syndromes of the model were evaluated by observing the changes of general condition, electrocardiogram and myocardial microcirculation. Results: compared with the normal group, the rats in the model group showed signs such as lip circumference, purple color of paws, hypothermia, electrocardiogram (ECG) showed that ST segment was significantly elevated, heart rate slowed down, and so on. Myocardial microcirculation test showed that myocardial microvein contracted, red blood cell velocity decreased significantly, and plasma TXB2/6-Keto-PGF1 伪 increased significantly (P0.05 or P0.01). Conclusion: the animal model of cold coagulation and blood stasis syndrome of coronary heart disease angina pectoris was established successfully through the analysis of macroscopic characterization and microscopic index of the model. The model of syndrome combination based on western medicine is more objective and reliable, which is more suitable for clinical diagnosis and treatment.
【作者单位】：北京中医药大学第一临床医学院;解放军总医院中医研究所;
【基金】：国家重点基础研究发展计划(973计划)资助项目-基于微血管病变性疾病的营卫“由络以通、交会生化”研究(2012CB518600)
【分类号】：R-332

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