肺炎克雷伯菌单基因分子进化分析及多位点序列分型与基因组系统学研究

发布时间：2019-08-16 11:23

【摘要】：目的比较7株已完成全基因组测序的肺炎克雷伯菌单基因分子进化分析及多位点序列(MLST)分型与基因组系统学研究的结果,研究其亲缘关系。方法采用Roche 454高通量测序技术对1株泛耐药肺炎克雷伯菌JM45株做全基因组测序(完成图),再与NCBI已完成全基因组测序的6株肺炎克雷伯菌9种看家基因进行单个基因分子进化分析,将该9个基因序列串联作MLST分型,并将7株肺炎克雷伯菌的全基因组序列作聚类分析(为Fast Minimum Evolutin法)。结果 JM45株测得一条完整的基因组(染色体)序列及两条质粒序列;因为不同的基因进化率不同,7株肺炎克雷伯菌9种看家基因单个基因序列的分子进化图并不一致;7株肺炎克雷伯菌MLST分型可见JM45株与HS11286株相同(即为同一型),1084株与NTUH-K2044株相同,但7株肺炎克雷伯菌基因组系统学分析可见,JM45株与HS11286株并不相同(即非为同一型),JM45株与HS11286株距离最近,与342株距离最远,其他各株介于二者之间。结论采用单个基因序列的分子进化分析法及MLST分型用作菌株亲缘性关系分析法并不可靠,本研究为国内首个单个看家基因分子进化分析及MLST分型与基因组系统学研究的比较报道。
[Abstract]:Objective to compare the results of single gene molecular evolution analysis, multi-site sequence (MLST) typing and genomic systematics of 7 strains of Klebsiella pneumoniae that have completed genome sequencing, and to study their genetic relationship. Methods the whole genome of a pan-resistant Klebsiella pneumoniae JM45 strain was sequenced by Roche 454. the single gene molecular evolution analysis was carried out with 9 housekeeping genes of 6 strains of Klebsiella pneumoniae that had completed the whole genome sequencing of NCBI. The 9 gene sequences were tandem for MLST typing, and the whole genome sequences of 7 strains of Klebsiella pneumoniae were analyzed by cluster analysis (Fast Minimum Evolutin method). Results A complete genome (chromosome) sequence and two plasmid sequences were obtained by JM45 strain, and the molecular evolution map of single gene sequence of 9 housekeeping genes of 7 strains of Klebsiella pneumoniae was not consistent because of the different gene evolution rates. The MLST typing of 7 strains of Klebsiella pneumoniae showed that JM45 strain was the same as HS11286 strain (that is, the same type), 1084 strains were the same as NTUH-K2044 strain, but 7 strains of Klebsiella pneumoniae genome systematic analysis showed that JM45 strain was not the same as HS11286 strain (that is, not the same type), JM45 strain was the closest to HS11286 strain and farthest from HS11286 strain, while the other strains were between the two strains. Conclusion it is not reliable to use molecular evolution analysis of single gene sequence and MLST typing to analyze the affinity relationship of strains. This study is the first comparative report on molecular evolution analysis of single housekeeping gene and MLST typing and genomic systematics in China.
【作者单位】：杭州市余杭区中医院检验科;南通大学附属第三医院检验科;宁波市第一医院检验科;浙江大学医学院附属第一医院传染病诊治国家重点实验室;
【基金】：国家“十一五”科技重大专项基金资助项目(2008ZX100-03-004) 杭州市科技计划引导基金资助项目(2012027) 杭州市卫生科技计划基金资助项目(2010B078) 杭州市余杭区重大科技基金资助项目(2012001)
【分类号】：R378.996

【共引文献】