百日咳鲍特菌红霉素耐药性及耐药相关分子特征分析

发布时间：2017-12-26 17:18

本文关键词：百日咳鲍特菌红霉素耐药性及耐药相关分子特征分析　出处：《中国疫苗和免疫》2016年02期 　论文类型：期刊论文

【摘要】：目的分析百日咳鲍特菌(Bordetella pertussis,Bp)红霉素耐药性并对其耐药相关分子特征进行检测。方法对分离自西安市2014年百日咳报告病例中的Bp通过E-Test检测其红霉素耐药性,通过聚合酶链反应(PCR)和PCR-测序方法分别检测可能出现的耐药基因及耐药相关位点改变。结果 10株Bp临床株分布于西安市6个区县,对红霉素的最小抑菌浓度(MIC)均256μg/ml,所有菌株都出现23S rRNA基因的A2047G突变,未见其他耐药基因及位点改变。结论西安市的红霉素耐药Bp已较为普遍,23S rRNA基因的A2047G突变可能是其最主要的耐药机制。应在百日咳监测的同时加强病原的抗生素耐药性监测及耐药相关分子的检测。
[Abstract]:Objective to analyze the resistance of Bordetella pertussis (Bp) to erythromycin and to detect the molecular characteristics of its drug resistance. Methods Bp was isolated from the reported cases of pertussis in Xi'an in 2014. Its erythromycin resistance was detected by E-Test. The possible resistance genes and drug-resistant related sites were detected by polymerase chain reaction (PCR) and PCR- sequencing. Results 10 clinical isolates of Bp were distributed in 6 districts and counties of Xi'an, and the minimum inhibitory concentration (MIC) of erythromycin was 256 g/ml. All the strains showed A2047G mutation of 23S rRNA gene, and no other resistance genes and loci were changed. Conclusion the erythromycin resistant Bp in Xi'an is more common, and the A2047G mutation of the 23S rRNA gene may be the most important mechanism of drug resistance. It is necessary to strengthen the monitoring of antibiotic resistance and the detection of drug resistance related molecules at the same time of pertussis monitoring.
【作者单位】：西安市疾病预防控制中心;
【基金】：陕西省自然科学基金项目(编号2015JM8391)
【分类号】：R516.6;R440
【正文快照】： 百日咳是中国法定报告乙类传染病,目前尚未发现除人之外的其他宿主,百日咳鲍特菌(Bordetellapertussis,Bp)是引起百日咳最主要的病原菌[1]。以红霉素为代表的大环内酯类抗生素是治疗百日咳及预防性服药的首选抗生素。全球首株红霉素耐药的Bp于1994年发现于美国,并引起了广泛关

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