梅毒血清学检测3种方法性能及适用性比较

发布时间：2017-12-31 11:09

本文关键词：梅毒血清学检测3种方法性能及适用性比较　出处：《齐鲁医学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的评价3种梅毒血清学检测方法的性能,对其适用性进行探讨。方法应用甲苯胺红不加热血清试验(TRUST)、酶联免疫吸附试验(TP-ELISA)和颗粒凝集试验(TPPA),分别对194例梅毒病人和145例非梅毒病人进行平行检测,比较3种方法检测梅毒的阳性检出率、灵敏度、特异度、阳性预测值和阴性预测值。结果TRUST对潜伏期梅毒及一期梅毒的检出率分别为73.33%和75.93%,均低于TP-ELISA和TPPA,差异有统计学意义(χ2=7.03、16.15,P0.05);TP-ELISA和TPPA对各期梅毒的检出率比较,差异无统计学意义(P0.05)。145例非梅毒病人中,TRUST、TP-ELISA、TPPA分别检出8例、2例和0例阳性,假阳性率分别为5.52%、1.38%和0,差异有显著性(χ2=9.40,P0.05)。TP-ELISA及TPPA检测梅毒的灵敏度高于TRUST,差异有统计学意义(χ2=18.17,P0.01);TPPA检测梅毒特异度、阳性预测值、阴性预测值均高于TRUST,差异有统计学意义(χ2=2.96~17.04,P0.05)。结论 3种方法各有适用性,选用合理的检测方法可为梅毒的诊断提供更准确的参考依据。
[Abstract]:The performance of the 3 detection methods of syphilis serology for the purpose of evaluation, and discusses its applicability. Methods using toluidine red unheated serum test (TRUST), enzyme linked immunosorbent assay (TP-ELISA) and particle agglutination test (TPPA), were used to detect 194 cases of syphilis patients and 145 cases of non syphilis patients, comparison 3 methods for detecting syphilis positive rate, sensitivity, specificity, positive predictive value and negative predictive value. Results TRUST of latent syphilis and syphilis detection rates were 73.33% and 75.93%, were lower than those of TP-ELISA and TPPA, the difference was statistically significant (2=7.03,16.15, P0.05); TP-ELISA and TPPA on the rate of comparison of detection of syphilis, there was no statistically significant difference (P0.05).145 cases of non syphilis patients, TRUST, TP-ELISA, TPPA were detected in 8 cases, 2 cases and 0 cases of positive, false positive rates were 5.52%, 1.38% and 0, there was significant difference (x 2=9.40, P0.0 5) sensitivity of.TP-ELISA and TPPA detection of syphilis was higher than TRUST, the difference was statistically significant (2=18.17, P0.01); TPPA detection of syphilis specificity, positive predictive value, negative predictive value was higher than TRUST, the difference was statistically significant (2=2.96~17.04, P0.05). Conclusion the 3 methods have different applicability, selecting reasonable testing methods can provide more accurate reference for the diagnosis of syphilis.

【作者单位】：清河县人民医院检验科;
【分类号】：R759.1;R446.6
【正文快照】： 梅毒是由苍白螺旋体即梅毒螺旋体引起的一种慢性性传播疾病,主要通过性途径传播。近年来,其感染率和发病率增长趋势明显[1-3],越来越引起社会各界的广泛关注。对梅毒病人进行早诊断、早治疗是控制其传播蔓延的重要环节。选择适宜的检验方法有助于梅毒的早期诊断,血清学方法是

【相似文献】