慢性乙型肝炎及后期肝病患者外周血T淋巴细胞亚群标志的研究

发布时间：2018-01-05 23:16

本文关键词：慢性乙型肝炎及后期肝病患者外周血T淋巴细胞亚群标志的研究　出处：《实用预防医学》2016年07期 　论文类型：期刊论文

【摘要】：目的探讨慢性乙型肝炎及其后期肝病患者免疫状态,了解其变化及临床意义。方法纳入临床确诊的慢性乙型肝炎(chronic hepatitis B,CHB)患者37例、乙型肝炎后肝硬化(liver cirrhosis,LC)患者35例、乙肝肝癌(hepatocellular cancer,HCC)患者32例及健康对照(healthy controls,HC)38例。流式细胞术检测其外周血T淋巴细胞亚群(CD3~+、CD3~+CD4~+、CD3~+CD8~+、CD4~+/CD8~+和Th1、Th2)的构成比,用ELISA法分析各组血清中Th1类(IFN-γ)、Th2类(IL-4)、Th17(IL-17)细胞因子的表达量。结果 CHB、LC、HCC与正常对照相比外周血CD3~+、CD3~+CD4~+、CD3~+CD8~+、CD4~+/CD8~+百分数比例均降低,差异均有统计学意义(P0.05或P0.01);CHB、LC、HCC患者TH1(CD4~+IFN-γ+)细胞频率明显低于健康对照(P0.05),CHB、LC、HCC三组患者TH2(CD4~+IL4~+)均高于健康对照(P0.05);对应CHB、LC、HCC患者血清IL-4、IL17明显高于健康对照组(P0.05),而CHB、LC、HCC患者血清中IFN-γ低于健康对照组(P0.05)。慢性乙型肝炎患者病程与淋巴细胞亚群、细胞因子均有明显相关性(均P0.05)。结论慢性乙型肝炎、乙肝肝硬化及肝癌患者外周血存在T细胞亚群标志改变,提示患者免疫功能紊乱,监测免疫功能并纠正免疫紊乱对指导临床有重要意义。
[Abstract]:Objective to investigate the chronic hepatitis B patients with liver disease and late immune status, understand the changes and the clinical significance. Methods in clinical diagnosis of chronic hepatitis B (chronic hepatitis, B, CHB) in 37 patients with hepatitis B cirrhosis (liver, cirrhosis, LC) in 35 patients with hepatitis B liver cancer (hepatocellular, cancer, HCC) and 32 cases of health control group (healthy controls, HC) in 38 cases. Flow cytometry peripheral blood T lymphocyte subsets (CD3~+, CD3~+CD4~+, CD3~+CD8~+, CD4~+/CD8~+ and Th1, Th2) the proportion of class Th1 in the serum of each group by ELISA method (IFN-), Th2 (IL-4), Th17 (IL-17) expression of cytokines. The results of CHB, LC, HCC and normal controls peripheral blood CD3~+, CD3~+CD4~+, CD3~+CD8~+, CD4~+/CD8~+ percentage ratio decreased, the differences were statistically significant (P0.05 or P0.01); CHB, LC, HCC in patients with TH1 (CD4~+IFN- +) cells significantly lower frequency In healthy control (P0.05), CHB, LC, HCC three groups of patients with TH2 (CD4~+IL4~+) were higher than those in healthy controls (P0.05); the corresponding CHB, LC, HCC in serum of patients with IL-4, IL17 was significantly higher than the healthy control group (P0.05), CHB, LC, HCC in blood serum of patients with IFN- were lower than the healthy control group (P0.05). Chronic hepatitis B patients with lymphocyte subsets, cytokines were significantly correlated (P0.05). Conclusion the patients with chronic hepatitis B, liver cirrhosis and hepatocellular carcinoma exist in peripheral blood of patients with signs of T cell subsets change, suggesting that the immune function of patients with disorder of immune function, monitoring and correcting the immune disorder has important significance for guiding clinical practice.

【作者单位】：中南大学湘雅医学院医学检验系;天津金域医学检验所;天津市第三中心医院;武警8642部队卫生队;
【分类号】：R512.62;R446.6
【正文快照】： 2.天津金域医学检验所;3.天津市第三中心医院;4.武警8642部队卫生队乙肝病毒感染的致病机理很复杂。已知,从慢性乙肝、肝硬化发展至肝癌患者体内存在免疫功能紊乱[1]。T淋巴细胞主要参与人体细胞免疫,持续性乙肝病毒(hepatitis B virus,HBV)感染和形成肿瘤过程中,体内T淋巴细

【相似文献】